复合益生菌粉对刺参生长·体壁成分和消化酶活性的影响

Effects of Compounnd Probiotics Powder on the Growth Performance,Body Wall Composition and Digestive Enzyme Activity of Sea Cucumber

[目的]研究饲料中添加复合益生菌粉对刺参生长、体壁成分和消化酶活性的影响。[方法]在基础日粮中分别添加含有0、0.2%、0.4%、0.6%、0.8%的复合益生菌粉(由枯草芽孢杆菌、嗜酸乳杆菌、酿酒酵母菌组成,含有106CFU/g)进行喂养试验,养殖8周。[结果]随着复合益生菌添加量的提高,刺参增重率和特殊生长率有增加趋势。与对照组相比,添加0.8%的复合益生菌能显著提高刺参的增重率和特定生长率(P<0.05)。复合益生菌对刺参成活率和体壁成分均无显著影响(P>0.05)。复合益生菌对刺参肠道的消化酶活性也无显著影响(P>0.05),但有提高刺参肠道脂肪酶活性的趋势。当益生菌添加量为0.4%时,刺参脂肪酶活性最高。[结论]复合益生菌粉能够促进刺参的生长。当益生菌添加量为0.8%时刺参的生长较好。

[Objective] The experiment was conducted to investigate the effects of compound probiotics powder on the growth,body wall composition and digestive enzyme activity of sea cucumber,Apostichopus japonicus.[Method] Five diets were formulated by supplementing with 0. 0%（ control）,0. 4%,0. 6% and 0. 8% compound probiotics（ Bacillus subtilis,Lactobacilli acidophilus,Saccharomyces cerevisiae,106 cfu / g） in basal diet,respectively. The experiment lasted for 8 weeks.[Result]Weight gain and specific growth rate had an increasing trend with increasing compound probiotics,weight gain and specific growth rate in sea cucumber fed the diets with 0. 8% supplementation were significantly higher than that of control group（ P〈 0. 05）. There were no significant difference on survival rate and body wall composition among treatments（ P〈 0. 05）. Although there were no significant difference on digestive enzyme activity（ P〈 0. 05）,sea cucumber fed the diets with compound probiotics showed higher trends of lipase activity than those fed the control diet. The highest lipase activity appeared in 0. 4% supplementation group.[Conclusion]Diet supplementing with 0. 8% compound probiotics can promote the growth performance of sea cucumber.