昆仑雪菊对结肠癌细胞株体外抗肿瘤作用研究

Study on the Antitumor Activity of Colorectal Cancer Cells in vitro from Coreopsis Ticntoria Flowers of Kunlun Mountain

[目的]探讨昆仑雪菊中总黄酮粗提取物、纯化物及总多糖对人结肠癌细胞株(HCT116细胞、CACO-2细胞)增殖的抑制作用。[方法]体外培养结肠癌细胞,采用不同浓度昆仑雪菊中总黄酮粗提取物、纯化物及总多糖对其进行干预,MTT法检测细胞生长抑制率,流式细胞仪测细胞凋亡率。[结果]MTT法检测昆仑雪菊中总黄酮粗提取物、纯化物及总多糖对结肠癌细胞株的抑制作用具有浓度和时间依赖性;流式细胞仪检测昆仑雪菊总黄酮纯化物能增强结肠癌细胞株的凋亡率,且剂量与凋亡率呈正相关。[结论]昆仑雪菊总黄酮纯化物抗肿瘤活性优于总黄酮粗提物及总多糖,且对结肠癌细胞株的增殖有明显抑制作用,其作用机制可能与增强细胞凋亡有关。

[Objective]To investigate the anti- proliferative effect of the total flavonoids,the total polysaccharides from Coreopsis Ticntoria flowers of Kunlun mountain on the colorectal cancer cells. [Method] The colorectal cancer cells were cultured in vitro. The total flavonoids and the total polysaccharides of Coreopsis Ticntoria flowers interene the cells. The viability of cells was measured by MTT. The cell apoptosis was detected with flow cytometry. [Result] Different concentrations of the total flavonoids and the total polysaccharides were cytotoxic to cells in a time and dose dependent manner. The purified extracts of the total flavonoids could enhance apoptisis rate of the cells,and dose and apoptosia rate were positively correlated. [Conclusion] The anti- proliferative effect of purified extracts of the total flavonoids on the cells was stronger than the crude extract of the total flavonoids and the total polysaccharides. The mechanism may be related to the inhibition apoptisis rate of the cells.