砂梨抗枝干轮纹病鉴定方法及群体抗性评价

Artificial Inoculation Method of Trunk Ring Rot Disease of Pear and the Resistance Identification of F1Generation Group

[目的]建立砂梨抗枝干轮纹病的鉴定方法。[方法]采用轮纹病菌菌丝块、分生孢子悬浮液分别以离体和活体接种砂梨1年生枝条,比较发病时间、发病率、调查时间和接种工作量,并采用菌丝块针刺接种活体枝条的方法对德胜香×西子绿的223株F1代群体进行抗性评价。[结果]以菌丝块针刺法接种活体枝条发病率高、接种效果最好,可充分评价梨树对枝干轮纹病的抗性。采用菌丝块针刺接种活体枝条的方法,德胜香×西子绿的223株F1代群体中高抗单株有33株,抗性单株有41株,中抗单株有83株,感病单株有31株,高感单株有35株。[结论]试验结果为砂梨抗枝干轮纹病育种提供了参考。

[Objective] The aim was to establish one identification method for trunk ring rot disease of sand pear. [Method] The resistant evaluation of individual plant was inoculated with mycelia disc and spore suspension in vitro and in vivo,and disease time,disease rate,investigation method and inoculated work amount were compared. The resistance identification of 223 F1 plants of Deshengnxiang × Xizilv was identified with mycelia disc in vivo. [Result] The disease incidence and index with mycelia disc was higher than that of the spore suspension,and the disease incidence and index of in vivo was also higher than that of in vitro,which suggested that inoculation with mycelia disc in vivo was fit for resistance identification of trunk ring rot disease of sand pear. Among 223 F1 plants of Deshengnxiang × Xizilv,33 F1 plants were high resistant; 41 plants were resistant; 83 plants were middle resistant,31 plants were sensitive and 35 plants were high sensitive. [Conclusion]The results provide reference for resistant breeding of sand pear to trunk ring rot disease.