鱼肉制品中脂肪的测定

Fat Determination of Fish Products

鱼肉制品的脂肪含量较低且富含磷脂等结合态脂肪,鱼肉制品中的脂肪检测尚无国家标准。传统的脂肪检测方法无法准确地测定鱼肉制品中的脂肪含量。脂肪检测方法有很多种,每种方法都有其适用范围和优缺点。采用氯仿-甲醇法对比传统的酸水解法测定鱼肉制品中的脂肪含量。结果表明,氯仿-甲醇法弥补了酸水解法水解磷脂的不足,能够准确地测定鱼肉制品中的脂肪含量,在重复性条件下获得的2次独立测定结果的绝对差值不超过算术平均值的5%。

Fish products have low fat content but rich in phospholipids and so on,we still have no national standards for testing fat content of fish products. Traditional fat testing methods can not determine fat content of fish products accurately. There are many fat testing methods,each method has not only its scope but also advantages and disadvantages. In this paper,we compare chloroform- methanol method to the traditional measurement of acid hydrolysis for testing fat content of fish products. The results show that chloroform- methanol method make up the shortage of acid hydrolysis that hydrolyzing of phospholipids,and determine fat content of fish products accurately,under repeatable conditions,independent determination results show that absolute difference is no more than 5% of the arithmetic mean.