佛手的抗菌抗氧化研究

Study on antibacterial and antioxidant activities of extraction from C.m.var.sarcodactylis

目的了解佛手对金黄色葡萄球菌、大肠埃希菌等8种常见致病菌的抗菌作用及其抗氧化性能。方法分别用80%的甲醇水提取新鲜成熟的佛手及其烘干制品的酚类物质。采用微量稀释法,测定其提取液对金黄色葡萄球菌、大肠埃希氏菌、鼠伤寒沙门氏菌、福氏志贺氏菌、铜绿假单胞菌、蜡样芽孢杆菌、表皮葡萄球菌及白色念珠菌8种常见致病菌的最小抑菌浓度及最小杀菌浓度,并对其抗菌活性进行评价;采用紫外可见分光光度计测定其氧化还原能力及清除自由基能力,对其抗氧化活性进行评价。结果佛手提取液对革兰阳性菌的抗菌作用强于革兰阴性菌。烘干后的佛手提取液的抗氧化作用强于新鲜佛手。结论佛手对常见的致病菌有较强的抗菌作用,可以开发其成为新型天然抑菌剂。

Objective To understand the antibacterial activity of extract of C.m.var.sarcodactylis.against 8kinds of common pathogenic bacteria and its antioxidant activity.Methods The phenolic substances from fresh and dried C.m.var.sarcodactylis.were extracted by 80% methanol.The minimum inhibitory concentration（MIC）and minimum bactericidal concentration（MBC）of the extracting solution against 8kinds of target pathogens involving Staphylococcus aureus,Escherichia coli,Salmonella typhimurium,Shigella flexneri,Bacillus cereus,Pseudomonas aeruginosa,Staphylococcus epidermidis and Monilia albican were determined and its antibacterial activities were evaluated by using microdilution broth method;FRAP and TEAC were also determined and its antioxidant activities was evaluated by using ultraviolet and visible spectrophotometer.Results The antibacterial effect of the extracting solution from C.m.var.sarcodactylis.against Gram-positive bacteria was stronger than that against Gram-negative bacteria.The antioxidant effect of the extracting solution from dried C.m.var.sarcodactylis.was stronger than that of the fresh.Conclusion C.m.var.sarcodactylis has strong antibacterial effect on common pathogenic bacteria and can be developed to become a new type of natural bacteriostatic agent.