血管生成素-2与大肠癌淋巴管密度的相关性研究

目的通过检测大肠癌标本的血管生成素-2（ANG-2）mRNA的表达，并进行微淋巴管计数，探讨ANG-2mRNA的表达与微淋巴管密度（MLVD）的关系，推断其在犬肠癌微淋巴管生成中的作用。方法应用RT-PCR检测癌组织、良性病变及手术切缘正常组织的ANG-2mRNA的表达；应用免疫组化技术，以LYvE．1标记微淋巴管并计数。结果ANG-2mRNA在癌组织内阳性表达64．O％，良性病变内阳性表达8.3％，手术切缘正常组织内阳性表达7．5％。ANG．2mRNA表达阳性的癌旁MLVD比阴性高（P〈0．01），且ANG-2mRNA的表达水平与癌旁MLVD存在相关性（P〈0．05，r=0．493）。癌旁MLVD及手术切缘正常组织MLfVD比癌组织高（P〈0．01），且癌旁MLVD比手术切缘正常组织MLVD高（P〈0．01）。Duke’s A、B期的癌旁MLVD较C、D期低；有淋巴结转移的癌旁MLVD比无淋巴结转移的癌旁MLVD高（P〈0．05）。结论ANG．2mRNA在大肠腺癌组织内有高表达并与癌旁MLVD的表达水平密切相关。

Objective Expression of angiopoietin-2mRNA was detected and microlymphatic vessel density （ MLVD ） was counted in CRC, then investigated correlation of the expression of ANG-2mRNA with MLVD in order to deduce relation of ANG-2 with lymphangiogenesis in CRC indirectly. Methods RT-PCR was applied to detect expression level of ANG-2mRNA in cancer tissue, benign tissue and normal surgical margin tissue, and MLVD marked by LYVE-1 was counted by morphometric. Results （ 1 ） In all samples, positive rates of ANG-2mRNA in CRC＇s cancer tissue, benign tissue and normal surgical margin tissue were （ 32/50 ） , （ 1/12 ） and （ 3/50 ） respectively. （ 2 ） There were 32 samples that ANG-2mRNA were positive, and among these samples peritumoral MLVD was higher than that in negative group （ P〈0.01 ） ; there were a correlation between ANG-2mRNA expression level and peritumoral MLVD （ P〈0.05, r=0.493 ） . （ 4 ） MLVD of peritumoral and normal surgeral margin＇s were higher than intratumoral MLVD （ P〈0.01 ） , peritumoral MLVD was higher than normal surgeral margin＇s MLVD additional （ P〈0.01 ） . （ 5 ） level of peritumoral MLVD in Duke＇s A-B stages was lower than C-D stages of CRC （ P〈0.05 ） ; MLVD with lymphatic metastasis was higher than without lymphatic metastasis （ P〈0.05 ） .Conclusions ANG-2 was high expressed in CRC, ANG-2 expression levels have closely relation with peritumoral MLVD.