激素和非生物胁迫对花生叶片白藜芦醇及其代谢产物合成的影响

Resveratrol and its metabolites accumulation responding to abiotic stresses and hormones in peanut seedlings

花生中白藜芦醇含量较低,但逆境可诱导大量白藜芦醇的积累。通过3种激素[水杨酸(SA)、乙烯利(ETH)、茉莉酸甲酯(Me JA)]和3种非生物胁迫[UV-C、H2O2、百草枯(PQ)]的方式在不同条件下进行诱导。结果表明:避光放置有利于UV-C处理后的花生叶片中白藜芦醇及其代谢产物的合成,4种芪化物(Res、PS、ε-viniferin、δ-viniferin)总含量为38.17μg/gFW;不避光(光照和黑暗交替16/8h)放置有利于H2O2、PQ、SA、ETH和Me JA处理后白藜芦醇及其代谢产物的合成,这5种处理芪化物总含量分别为32.76、32.21、0.49、2.42和1.42μg/gFW。在适当的剂量范围内,花生叶片中白藜芦醇及其代谢产物的积累随剂量的增加而增加,最佳的处理剂量分别为2.0h UV-C,1mmol/L PQ、1%H2O2、10mmol/L SA、50mmol/L ETH和50mmol/L Me JA;4种芪化物中以Res含量增加较为显著,而代谢产物的含量较低。研究表明,激素或非生物胁迫可诱导花生叶片中白藜芦醇及其代谢产物的积累,花生可作为一种获取白藜芦醇及其代谢产物的植物源。

Peanut synthesizes resveratrol is low in natural plants,but can be accumulated in great amount un-der stresses. Resveratrol and its metabolites（pterostilbene,ε - viniferin,δ - viniferin）in peanut seedlings were in-vestigated after hormone[salicylic acid（SA）,ethephon（ETH）,and methyl jasmonate（MeJA）]induction and abiotic（UV - C,H2 O2 ,and paraquat）stress. Results showed that leaves resveratrol was conducted after UV - C radiation under dark condition,and the total content of resveratrol and 3 metabolites were 38. 17μg / gFW . However, the above substance was better conducted after H2 O2 ,paraquat,salicylic acid,ethephon and methyl jasmonate treatments under 16 / 8h of light and dark alternation condition. The total contents were 32. 76,32. 21,0. 49,2. 42 and 1. 42μg / gFW respectively. Resveratrol and its metabolites increased with the treatment dose in peanut leaves, and reached the peaks with 2. 0h UV - C,1mmol/ L PQ,1% H2 O2 ,10mmol/ L SA,50mmol/ L ETH and 50mmol/ L MeJA. The increasement of resveratrol was significantly higher than those of 3 metabolites. The results suggested that peanut can be used as resveratrol source,especially after induced by the above factors.