MPB64免疫胶体金检测法在分枝杆菌菌种鉴定中的应用

A study on the application of MPB64 antigen detection of immune colloidal gold method for identification of Mycobacterium

目的评价MPB64免疫胶体金快速检测法在分枝杆菌菌种鉴定中的应用价值。方法以PCR扩增测序法为"金标准",对MPB64免疫胶体金法和传统PNB/TCH法对2种分枝杆菌标准菌株和418株临床分枝杆菌分离株的菌种鉴定能力作比较。结果胶体金法、PNB/TCH法与PCR测序法结果一致率分别是95.93%和87.08%,胶体金法鉴定结核分枝杆菌敏感度、特异度、阳性预测值和阴性预测值分别88.41%、99.64%、99.19%和94.58%,而PNB/TCH法为62.32%、99.29%、97.72%和84.24%。胶体金法15 min即可作出鉴定结果,较传统的PNB/TCH法提前了28 d,比测序法提前3 d。结论与PNB/TCH法相比,MPB64免疫胶体金法在分枝杆菌菌种鉴定中,具有较高的特异度和灵敏度,且方法简便、快速,适合基层推广应用。

Objective To know the application of MPB64 antigen detection of immune colloidal gold method for identification of Mycobacterium. Methods Taking the PCR amplified sequencing method as the ＇ gold standard＇,a total of418 clinical isolated mycobacterium tuberculosis and 2 standard strains were tested by immune colloidal gold method,and was compared with the traditional method of PNB / TCH. Results Compared with sequencing method,the coincidence rate of immune colloidal gold method and PNB / TCH identification test was 95. 93% and 87. 08% respectively. The sensitivity,specificity,positive predictive value（ PPV） and negative predictive value（ NPV） of immune colloidal gold method identification were 88. 41%,99. 64%,99. 19% and 94. 58% respectively. It costs MPB64 immune colloidal gold method15 minutes to carry out the results,less than 28 days of the traditional method,as well as 3 days of sequencing method.Conclusion Compared with PNB / TCH method,MPB64 immune antigen colloidal gold method has higher specificity and sensitivity,and could be applied in identification of Mycobacterium.