HPLC法同时测定复方血栓通胶囊中5种皂苷类成分

Determination of five saponins compounds in compound Xueshuantong capsule by HPLC

目的建立HPLC法同时测定复方血栓通胶囊中人参皂苷Rg1、Re、Rb1、Rd和三七皂苷R1含量的方法。方法采用YMC-Pack Pro C18柱（250 mm×4.6 mm,5μm）,以乙腈为流动相A,水为流动相B进行梯度洗脱,检测波长为203 nm,流速为1.0 m L·min^-1,柱温为35℃。结果测得三七皂苷R1在50.10-501.00 ng、人参皂苷Rg1在151.02-1 510.20 ng、人参皂苷Re在32.01-302.10 ng、人参皂苷Rb1在102.23-1 022.30 ng、人参皂苷Rd在16.32-163.20 ng范围内线性关系良好（r=0.999 9）;精密度、稳定性、重复性RSD均小于3%;平均加样收回率在95%-105%之间。结论该法简便、准确、重复性好,可用于复方血栓通胶囊的质量控制。

Objective To establish an HPLC method for determination of Notoginsenoside R1 ,Ginsenosides Rg1 ,Ginsenosides Re,Gin-senosides Rb1 ,Ginsenosides Rd in Compound Xueshuantong Capsule by HPLC.Methods The compounds were separated by gradient elution on YMC-Pack Pro C18 column（250 mm ×4.6 mm,5 μm）as stationary phase;acetonitrile and water were adopted as gradient mixed mobile phase.The detection wavelength was set at 203 nm and the column temperature was 35℃,with the flow rate of 1.0 mL· min ^-1 .Results Notoginsenoside R1 ,Ginsenosides Rg1 ,Ginsenosides Re,Ginsenosides Rb1 ,Ginsenosides Rd all had good linear rela-tion（r =0.999 9）in the ranges of 50.10 -501.00 ng,151.02 -1 510.20 ng,32.01 -302.10 ng,102.23 -1 022.30 ng,16.32 -163.20 ng,respectively.RSD of precision,stability and repeatability were lower than 3 %.The average recovery rates were in the range of 95% -105%.Conclusions The method is simple,accurate,reproducible and suitable for the quality control of Compound Xue-shuantong Capsule.