用GC-MS法测定大鼠血浆中1,8-桉树脑的浓度及其药动学研究

Concentration determination of 1,8-cineole in rat plasma by GC-MS method and its pharmacokinetics study

目的：建立大鼠血浆样品中1,8-桉树脑的定量分析方法,并测定其在大鼠体内的药动学参数。方法：以α-蒎烯为内标,采用GC-MS法,选择离子模式（m/z 93）,建立大鼠血浆中1,8-桉树脑的定量分析方法,并测定大鼠灌胃1,8-桉树脑200mg/kg后的药动学参数。结果：1,8-桉树脑与内标保留时间分别为3.13和3.91min;1,8-桉树脑经大鼠灌胃给药后的主要药动学参数如下：tmax为（0.75±0.16）h,cmax为（26.55±3.73）μg/ml,t1/2为（1.92±0.60）h,MRT为（3.22±0.97）h,AUC0-12h为（88.22±3.96）μg·h·ml^-1,AUC0~∞为（91.24±4.13）μg·h·ml^-1。结论：本方法灵敏度高、操作简单,可用于大鼠血浆中1,8-桉树脑的含量测定及药动学研究。

Objective：To establish the quantitative analysis method of 1,8-cineole in rat plasma and determine its pharmacokinetic parameters in vivo.Methods：Quantitative analysis method for 1,8-cineole in rat plasma was established by using GC-MS,with selected ion mode（m/z93）andα-pinene as internal standard.Pharmacokinetic parameters of 1,8-cineole were studied after gavage 200mg/kg to the rats.Results：Retention time for 1,8-cineole and internal standardα-pinene were 3.13 and 3.91 min,respectively.The main pharmacokinetic parameters of 1,8-cineole after gavage were as follows：tmax（0.75±0.16）h,cmax（26.55±3.73）μg/ml,t1/2（1.92±0.60）h,MRT（3.22±0.97）h,AUC0-12h（88.22±3.96）μg·h·ml-1 and AUC0-∞（91.24±4.13）μg·h·ml-1.Conclusion：The proposed method is sensitive and simple,and could be used for the determination and pharmacokinetics study of 1,8-cineole.