摘要
为研究成骨细胞在Ti、Ti6Al4V和Ti6Al7Nb三种钛金属表面经喷砂酸蚀处理后的生物活性,通过Al2O3喷砂和盐酸、硫酸混合物酸蚀等表面改性处理技术,在Ti、Ti6Al4V和Ti6Al7Nb三种表面进行喷砂酸蚀处理,通过扫描电镜(scanning electron microscope,SEM)观察样品的表面形貌,用3D激光共聚焦显微镜测量样品表面的粗糙度和三维立体结构;将SD大鼠成骨细胞以1×105 cells/m L密度接种于Ti、Ti6Al4V和Ti6Al7Nb表面后通过MTT活性实验观察成骨细胞在样品表面的增殖,通过荧光显微镜观察成骨细胞在样品表面的黏附、生长与增殖。结果显示Ti、Ti6Al4V和Ti6Al7Nb在经过喷砂酸蚀处理后,表面均呈现出微米级多孔形貌,表面粗糙度增加;细胞在SLA处理后的Ti、Ti6Al4V和Ti6Al7Nb表面增殖良好,细胞伸展更为显著;其中又以Ti6Al7Nb表面的细胞数量最多。表明SLA的表面处理能够促进Ti、Ti6Al4V和Ti6Al7Nb的生物活性;经SLA处理的Ti6Al7Nb比Ti和Ti6Al4V表现出更好的生物学活性,成骨细胞在其表面呈现出更好的增殖及黏附能力。
In the present study, the in-vitro biological responses to Titanium(Ti) and two competitive titanium alloys(Ti6Al4V and Ti6Al7Nb) were investigated after modifying their surfaces by sandblasting and acid etching(SLA). The SLA process was sandblasted by Al2O3 particles and acid etched by a mixture of hydrochloric acid and sulfuric acid. The surface morphologies of samples were characterized by scanning electron microscope(SEM), the roughness and three-dimensional structure were detected by 3D laser scanning confocal microscope. Osteoblasts seeded on Ti, Ti6Al4 V and Ti6Al7 Nb surface were observed by MTT proliferation tests and Calcein-AM staining experiments. After the process of SLA, the surfaces of the samples were porous of micro meters. Even though micro structure of the samples exhibited somewhat similarly. Osteoblasts proliferation and adhesion on the surface of Ti6Al7 Nb alloy was greater than that of the Ti6Al4 V and Ti after SLA, which was significant for implant surface modification.
出处
《大连大学学报》
2015年第3期84-89,共6页
Journal of Dalian University
基金
辽宁省教育厅基金(2013481)
中国博士后科学基金(2014M551097)
辽宁省博士后启动基金(20141198)
大连科技计划项目基金(2013E11SF057)