高温胁迫对不同花生品种生理指标的影响

Effects of High Temperature Stress on Physiological Indexes of Different Peanut Varieties

为了比较不同花生品种对高温胁迫的耐性机制,以高温敏感性花生品种冀花4和抗性品种豫花9719为供试材料,在苗期进行45℃的高温处理16d,测定了生物量、光合作用及活性氧簇(Reactive oxygen species,ROS)代谢相关指标。结果表明,与对照相比,(1)高温处理后两品种株高和干重均降低,豫花9719和冀花4株高分别降低37.95%和37.04%;冀花4的干重降低21.17%,豫花9719的干重不变。(2)冀花4的Fv/Fm降低1.71%,但豫花9719未受影响;高温处理使豫花9719叶片含水量及叶绿素a的含量分别增加0.78%和33.89%,两品种叶片类胡萝卜素含量分别降低11.14%和13.06%,可溶性蛋白含量分别增加54.04%和70.25%。(3)高温处理后,豫花9719和冀花4叶片MDA含量分别增加39.30%和36.30%,SOD活性分别增加28.82%和30.84%,但CAT活性不变;豫花9719叶片中游离脯氨酸含量增加811.23%。由此得出结论,抗性品种豫花9719叶片中渗透调解物质游离脯氨酸含量的增加,有效维持了叶片组织内含水量,减轻了高温胁迫对PSII的伤害,可能是其抵御高温胁迫的有利生理反应。

To study the toleration of different peanut cultivars to high temperature stress, Yu-hua971 9 （sensitive to high temperature） and Jihua4 （tolerant to high temperature） were used as trial material.After the treatment of 45℃ for 1 6 days in seedling stage, the metabolism related indicators such as biomass, photosynthesis and reactive oxygen species （ROS） were measured.The results showed that： （1） By comparing with control group, high temperature treatment made the plant height and dry weight of both cultivars decreased, the plant height of Yuhua9719 and Jihua4 reduced by 37.95%and 37.04%, the dry weight of Jihua4 reduced 21.17% while Yuhua971 9 unchanged .（2） By compa-ring with the control, Fv /Fm of Jihua4 reduced 1.71% and Yuhua971 9 unaffected.The high temper-ature made the water content and chlorophyll of Yuhua971 9 increased 0.78% and 33.89% respective-ly.The carotenoids content of the two varieties decreased by 1 1.14% and 13.06% respectively, and the soluble protein content of the two varieties increased by 54.04% and 70.25% respectively.（3） After the high temperature treatment, MDA content of Yuhua971 9 and Jihua4 increased by 39.30%and 36.30% respectively, SOD activities increased by 28.82% and 30.84% respectively, but the CAT activities unchanged.Free proline content of Yuhua971 9 increased 81 1.23%.It showed that, the in-crease of free proline content in Yuhua971 9 effectively maintained the water content in leaf tissues and reduced the damage to PSII.This might be the Yuhua971 9＇s beneficial physiological responses to the high temperature.