Sysmex XE-5000全自动血液分析仪高荧光体液细胞计数在脑膜癌诊断中的应用

Application of high-fluorescence body fluid cell mode on the platform Sysmex XE-5000 automated blood analyzer in diagnosis of meningeal carcinomatosis

目的评价Sysmex XE-5000全自动血液分析仪高荧光体液细胞(HF-BF)计数性能,并分析其在脑膜癌诊断中的应用价值。方法通过精密度试验和方法学比较试验对HF-BF进行性能评价;回顾性分析厦门大学附属中山医院2010年6月至2012年9月295份脑脊液的HF-BF检测结果。结果 Sysmex XE-5000全自动血液分析仪HF-BF计数精密度高、与细胞学检查一致性好。脑膜癌组每百个白细胞检测到的高荧光体液细胞数(HF-BF%)高于其他脑疾病组,两两比较差异有统计学意义(P<0.05)。当HF-BF%为4.3%时,受试者工作特征(ROC)曲线下面积(AUC)为0.933,灵敏度为95.2%,特异度为92.7%,即HFBF%大于4.3%时,脑脊液检出肿瘤细胞的可能性大。结论 Sysmex XE-5000全自动血液分析仪HF-BF参数是脑膜癌早期诊断的有效参考指标,具有较好的临床应用价值。

Objective To evaluate the performance of high-fluorescence body fluid cell （HF-BF）mode on the platform Sysmex XE-5000 automated blood analyzer,and analyse its clinical application value in diagnosis of meningeal carcinomatosis.Methods E-valuated the performance of HF-BF by using precision test and methodology comparison test.Retrospectively analyzed 295 test re-sults of cerebrospinal fluid in Zhongshan Hospital Affiliated to Xiamen University from June 2010 to September 2012.Results HF-BF on the platform Sysmex XE-5000 automated blood analyzer had high precision,and exhibited a good consistency with cytolgical examination.The percentage of high-fluorescence body fluid cell（HF-BF%）in the meningeal carcinomatosis group was higher than that in other cerebral diseases groups,had statistically significant differences （P 〈 0.05 ）.The cut-off value for HF-BF% was 4.3%,while the area under a receiver operating characteristic （ROC）curve （AUC）was 0.933,the sensitivity was 95.2%,and the specificity was 92.7%.When HF-BF% was over 4.3%,it was more likely to detect tumor cell in cerebrospinal fluid cytology.Con-clusion HF-BF is an effective reference index for the early diagnosis of meningeal carcinomatosis and has significant clinical appli-cation value.