蛋白质组学技术分析小鼠中脑能量代谢蛋白

Proteomic analysis of energy metabolism-related proteins of mice midbrain

目的采用蛋白质组学技术鉴定小鼠中脑部位与能量代谢相关的蛋白,探讨这些蛋白与神经性疾病的关系。方法提取小鼠中脑蛋白质,采用双向电泳分离,切取蛋白点进行基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF MS)鉴定。结果双向电泳技术结合MALDI-TOF MS成功鉴定出24个与能量代谢相关蛋白。结论建立了小鼠中脑部位蛋白质的鉴定方法,为探讨这类蛋白质在神经系统疾病中的作用奠定了基础。

Objective To identify energy metabolism-proteins of mice midbrain by using the proteomic technique,and to investi-gate the relationship between these proteins and neural diseases.Methods Two-dimensional gel electrophoresis was used to sepa-rate totally soluble proteins extracted from mice midbrain.Some protein spots on two-dimensional gel electrophoresis gels were ana-lyzed by using matrix assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF MS）.Results 24 protein spots related with energy metabolism were separated by two-dimensional gel electrophoresis and identified by using MALDI-TOF MS successfully.Conclusion The establishment of energy metabolism-related proteins map of mice midbrain lays a foundation for the research on the involvement of these proteins in neural disease pathogenesis.