新疆地区男性不育患者人乳头瘤病毒感染和抗精子抗体的回顾性研究

Retrospective analysis of association between human papilloma virus infection and antisperm antibodies in male infertile patients in Xinjiang area

目的探讨新疆地区男性不育患者人乳头瘤病毒(HPV)感染与抗精子抗体(AsAb)的关系。方法选取127例男性不育患者作为试验组,130例正常生育男性作为对照组。采用反向斑点杂交方法检测HPV精液感染,采用酶联免疫吸附试验(ELISA)检测精液中AsAb水平。结果男性不育患者HPV感染率(67.7%)高于正常生育男性HPV感染率(16.2%),差异有统计学意义(P<0.05);HPV精液感染的男性不育患者,AsAb阳性率(38.4%)高于无HPV感染的不育男性(17.1%),差异具有统计学意义。与HPV感染阴性男性不育患者比较,HPV感染男性不育患者精子活率和(a+b)级活力均降低,精子畸形率升高,差异均有统计学意义(P<0.05)。结论该地区男性不育患者HPV感染率较高,HPV感染与AsAb阳性有关,可进一步导致男性不育。

Objective To explore the association between human papilloma virus（HPV）infection and antisperm antibodies（As-Ab）in male infertile patients in Xinjiang area.Methods A total of 127 cases of infertile male patients were selected as experimental group,other 130 cases of normal male were enrolled in the control group.HPV sperm infection was detected by using reverse dot blot hybridization kit.The levels of AsAbs in semen specimen were measured by using the enzyme-linked immunosorbent assay （ELISA）.Results The infection rate of male infertile patients（67.7%）was higher than that of normal male（1 6.2%）,with signifi-cant differences（P 〈0.05）.The positive rate of AsAb of male infertile patients with HPV infection（38.4%）was higher than that of male infertile patients without HPV infection（1 7.1%）,with significant differences（P 〈0.05）.Compared with male infertile pa-tients without HPV infection,the percentages of sperm motility and grade （a＋b）sperm motility were decreased in male infertile pa-tients with HPV infection,wihile the sperm malformation rate was increased in male infertile patients with HPV infection,with sig-nificant differences（P 〈0.05）.Conclusion The HPV infection rate of male infertile patients is relatively high in this area.HPV sperm infection could be associated with AsAb that may further reduce male fertility.