余甘子叶提取物对荷瘤小鼠外周血T淋巴细胞CD4^+、CD8^+亚群的影响

The Effect of Extract of Phyllanthus Emblica Leaves(PL) on CD4^+ and CD8^+ in Peripheral T-cell lymphocyte of Tumor-bearing Mice

[目的]观察余甘子叶提取物(PL)对荷瘤小鼠外周血T淋巴细胞CD4+、CD8+亚群的影响。[方法]建立H22荷瘤小鼠模型,灌胃给予余甘子叶提取物高、中、低剂量(1.982,0.991,0.496 g/kg),观察余甘子叶提取物对荷瘤小鼠外周血T淋巴细胞CD4+、CD8+亚群的影响。[结果]余甘子叶提取物高、中剂量(1.982,0.991 g/kg)能显著提高荷瘤小鼠T淋巴细胞及其CD4+亚群相对数量(P<0.05),上调CD4+/CD8+亚群比例(P<0.05)。[结论]通过提高T淋巴细胞及其CD4+亚群相对数量,使CD4+/CD8+比例接近正常值,此可能是余甘子叶提取物抗肿瘤的免疫学机制之一。

[ Objective ] To observe the effect of extract in PL on CD4＋ and CD8＋in peripheral T-cell lymphocyte of tumor-bearing mice. [ Methods ] Ha mice tumor-bearing model was established and treated with PL of high, medium and low dose （ 1.982,0.991,0.496 g/kg） by intragastric administration. The effect of PL on CD4＋and CD8＋in peripheral T-cell lymphocyte of tumor-bearing mice was observed. [Results] The extract in PL of high and medium doses （1.982,0.991 g/kg）could significantly improve the relative quantity of T lymphocyte and CD4＋ T lymphocyte of tumor-bearing mice （P〈0.05）and up-regulated the proportion of CD4 ＋ and CD8 ＋（P〈0.05）. [ Conclusion ] Improving the quantity of T lymphocyte and CD4＋ T lymphocyte of tumor-bearing mice can make the proportion of CD4＋/ CD8＋ T lymphocyte close to normal value. That is one of the anti-tumor immunological mechanisms of PL extract.