恩施州内外环境硒水平与慢性乙肝患者外周血淋巴细胞亚群改变的研究

The Effects of Selenium Concentration in Body and Environment on the Change of Peripheral Blood Lymphocyte Subsets of Patients with Chronic Hepatitis B in Enshi

目的探讨湖北省恩施土家族苗族自治州内、外环境硒水平与慢性乙型肝炎患者外周血淋巴细胞亚群改变的关系。方法在恩施州境内高硒区、富硒区和低硒区设立病例组和对照组,采集血液样品用于检测血硒及外周血淋巴细胞亚群,同时采集相应地区的土壤、粮食用于硒测定;硒的测定采用氢化物发生原子荧光光度法,流式细胞术检测外周血淋巴细胞亚群。结果高硒区土壤、大米、茶叶含硒量[(7.73±0.66)、(2.96±0.26)、(1.58±0.29)mg/kg]均高于富硒区[(4.76±0.92)、(2.36±0.37)、(1.54±0.34)mg/kg]和低硒区[(0.71±0.20)、(0.10±0.08)、(0.11±0.05)mg/kg]。高硒区和富硒区茶叶硒含量没有显著差异外,其他两两比较均有统计学意义(P<0.01)。高硒、富硒、低硒区的对照组血硒水平均高于同区慢性乙肝组血硒水平(P<0.05);与低硒区比较对照组和病例组血硒水平均低于高硒区和富硒区(P<0.05)。慢性肝炎患者外周血淋巴细胞计数明显低于正常对照组(P<0.01)。结论高硒、富硒、低硒区慢性肝炎患者血硒水平显著降低,慢性肝炎患者外周血淋巴细胞亚群的改变与内、外环境硒水平有一定关系。

Objective To explore the correlation between the change of peripheral blood lymphocyte subsets of patients with chronic hepatitis B and the selenium（ Se） concentration in body and environment in Enshi Tujia and Miao Autonomous Prefecture,Hubei,China.Methods Patient group composed of the patients with hepatitis B virus and control group consisting of people were set up in high-Se area,Se-rich area and low-Se area in Enshi,respectively.Blood samples were collected from different groups in different areas to detect the Se concentration and the amount of peripheral blood lymphocytes of blood.The soil and crops（ mainly rice and tea） were collected to measure the Se concentration in environment in the three types of areas.Hydride generation atomic fluorescence spectrometry was utilized to measure the content of Se element,while the peripheral blood lymphocyte subsets were detected by using flow cytometry.Results Se concentrations in soil（ 7.73±0.66 mg / kg）,rice（ 2.96±0.26 mg / kg） and tea（ 1.58±0.29 mg /kg） in high-Se area are higher than those in Se-rich area（ 4.76±0.92,2.36±0.37 and 1. 54±0.34 mg /kg） and low-Se area（ 0.71±0.20,0.10±0.08 and 0.11±0.05 mg / kg）,respectively. There is no obvious difference of Se concentration between the high-Se and Se-rich area,but the other comparisons are of statistical significance（ P〈0. 01）. The blood Se content in control group is higher than that in patient group in each（ P〈0. 05）. The blood Se contents in control group and patient group in low-Se area are lower than those in high-Se and Se-rich area,respectively（ P〈0.05）. In high-Se and low-Se area,the counts of peripheral blood lymphocytes in the patients with chronic hepatitis are evidently lower than those in control group（ P〈0.01）,but in Se-rich area the change of the peripheral blood lymphocyte subsets is not as obvious as in high-Se and Se-rich area. Conclusion The blood Se contents of the patients with chronic hepatitis in high-Se,Se-rich and low-Se area decrease obviously in comparison with the healthy people.The change of the peripheral blood lymphocyte subsets in the patients with chronic hepatitis is found to be related to the Se concentration in body and environment.