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核桃NBS类抗病基因的克隆及序列分析 被引量：2

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摘要 NBS-LRR蛋白在植物抵御各种病原物的侵袭中发挥重要作用。利用RT-PCR技术以香玲核桃叶片为试材,克隆核桃核苷酸结合位点(nucleotide binding site,NBS)类抗病基因类似物(resistance gene analog,RGA),并对该基因和编码蛋白序列进行分析。结果表明,该序列长512 bp,具有NBS的典型结构域。利用DNAman软件进行相似性比对发现该序列与其他植物的NBS具有较高的同源性。核桃NBS类基因的克隆为其进一步研究应用奠定了基础。
作者 徐丽 陈新 魏海蓉 张力思 宗晓娟 王甲威 刘庆忠
机构地区 山东省果树研究所/山东省果树生物技术重点实验室
出处 《江苏农业科学》 北大核心 2015年第6期37-39,共3页 Jiangsu Agricultural Sciences
基金 科技部科技基础平台项目(编号:2014-048) 农业部种质资源保护与利用项目(编号:2014NWB009) 中俄国际科技合作专项(编号:2012DFR30700)
关键词 核桃 NBS-LRR 抗病基因 克隆 序列分析
分类号 Q785 [生物学—分子生物学]
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参考文献16

  • 1Johal G S, Briggs S P. Reductase activity encoded by the Hm/disease resistance gene Inmaize[ J]. Science, 1992,258 (5084) :985 - 987.
  • 2Martin G B,Bogdanove A J, Sessa G. Understanding the functions of plant disease resistance proteins [ J ]. Annual Review of Plant Biolo-gy,2003,54:23 - 61.
  • 3Martin G B, Brommonschenkel S H, Chunwongse J, et al. Map - based cloning of a protein kinase gene conferring disease resistance in tomato [ J ]. Science, 1993,262 (5138) : 1432 - 1436.
  • 4Yu Y G, Buss G R, Maroof M A. Isolation of a superfamily of candi- date disease -resistance genes in soybean based on a conserved nu- cleotide - binding site [ J ]. Proceedings of the National Academy of Sciences of the United States of America, 1996,93 (21) : 11751 - 11756.
  • 5Hommand - Kosack K E, Jones J D G. Plant diaease resistance gene [ J]. Annu Rev Plant Mol Biol, 1997,48:575 - 607.
  • 6Meyers B C, Kozik A, Griego A, et al. Genome -wide analysis of NBS - LRR - encoding genes in Arabidopsis [ J ]. The Plant Cell, 2003,15 (4) :809 - 834.
  • 7Monosi B, Wisser R J, Pennill L, et al. Full - genome analysis of re- sistance gene homologues in rice [ J ]. TAG. 2004,109 (7) :1434 - 1447.
  • 8Tameling W L, Elzinga S J, Darmin P S, et al. The tomato R gene products I - 2 and Mi - 1 are functional ATP binding proteins with ATPase activity[ J]. The Plant Ce11,2002,14:2929 -2939.
  • 9苏为耿,陆斌,刘金凤,赵平.短侧枝挂果核桃优良品种选择[J].广西林业科学,2012,41(3):248-251. 被引量：8
  • 10Deyoung B J, Innes R W. Plant NBS - LRR proteins in pathogen sensing and host defense [ J ]. Nature Immunology, 2006,7 ( 12 ) : 1243 - 1249.

二级参考文献82

  • 1黄代青.果树R基因同源序列克隆的初步研究[J].福建果树,2002,20(z1):1-4. 被引量：4
  • 2梁凤山,周春江,孔凡娜,王斌.桃基因组中R类抗病基因同源序列的克隆与序列分析[J].河北农业大学学报,2005,28(1):44-48. 被引量：5
  • 3[1]Johal GS, Brigg SP. Reductase activity encoded by the HM1 disease resistance gene in maize. Science, 1992, 258:985～987
  • 4[2]Hulbert SH,Webb CA,Smith SM,et al. Resistance gene complexes: evolution and utilization. Annu. Rev. Phytopathol., 2001, 39: 285-312
  • 5[3]Kanazin V, Marek LF, Shoemaker RC. Resistance gene analogs are conserved and clustered in soybean. Proc. Natl. Acad. Sci. USA 1996, 93:11746-50
  • 6[4]Yu YG, Buss GR, Maroof MSS. Isolation of a superfamily of candidate disease-resistance genes in soybean based on a conserved nucleotide-binding site. Proc. Natl. Acad. Sci. USA, 1996, 93:11751-11756
  • 7[5]Leister,D., A.Ballvora, F.Salamini, et al. A PCR-based approach for isolating pathgen resistance genes from potato with potential for wide application in plants. Nat. Genet, 1996, 14:421-429
  • 8[6]Arts M G, Lintel B, HolubE B, et al. Identification of R-gene homologous DNA fragments genetically linked to disease resistance loci in Arabidopsis thaliana. Mol Plant Microbe Interact, 1998, 11:251-258
  • 9[7]Collins NC, Webb CA, Seah S, et al. The isolation and mapping of disease resistance gene analogs in maize. Mol. Plant-Microbe Interact ,1998.11: 242-52
  • 10[8]Seah S,Sivasithamparam K,Karakousis A, et al. Cloning and characterization of a family of disease gene analogs from wheat and barley. Theor Appl Genet , 1998, 97:937-945

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江苏农业科学
2015年 第6期

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