针刺“头四关穴”对脑缺血再灌注家兔脑内单胺类神经递质及ATP酶的影响

Effect of Needling “Top siguan”on monoamine neurotransmitter and ATP enzyme in cerebral ischemia reperfusion rabbit

目的观察针刺"头四关穴"对家兔局灶脑缺血再灌注损伤后脑内单胺类神经递质及ATP酶含量的影响,探讨其对神经细胞保护的作用机制。方法将家兔随机分为假手术组、模型组和针刺组,每组又随机分为1 d、3 d和7 d 3个时间点。采用大脑中动脉线栓法(MCAO)建立家兔局灶性脑缺血再灌注模型,在脑缺血2 h后进行再灌注。针刺组家兔在脑缺血6 h后采用电针"头四关穴"进行治疗,每日1次。在再灌注后1 d、3 d和7 d分别处死取材。采用荧光分光光度法测定脑组织中多巴胺(DA)、去甲肾上腺素(NE)、5-羟色胺(5-HT)的水平;比色法检测缺血脑组织Na+-K+ATP酶、Ca2+ATP酶的含量。结果与假手术组比较,模型组脑组织中DA、NE和5-HT的水平明显降低,1 d时最为明显,随缺血再灌时间延长,上述指标水平有所提高;Na+-K+ATP酶和Ca2+ATP酶含量明显下降,差异有统计学意义(P<0.01)。与模型组比较,针刺组各时间点DA、NE和5-HT水平,以及Na+-K+ATP酶和Ca2+ATP酶含量均有不同程度的增加(P<0.01)。结论针刺"头四关穴"可有效改善脑缺血再灌注损伤家兔脑组织ATP酶代谢,减少脑缺血区单胺类神经递质的释放,抑制单胺类递质的神经毒性作用,从而发挥抗脑缺血损伤的作用。

Objective To observe the effect on the content of monoamine neurotransmitter and ATP enzyme by needling ＂Top siguan ＂in cerebral ischemia reperfusion rabbit,and to discuss the mechanism of nerve cells protection. Methods rabbits were randomly divided into three groups ： sham-operated group,model group and acupuncture group,and each group was divided into post-ischemia 1d,3d and 7d different periods subgroups. The focal cerebral ischemia and reperfusion model were established by the method of the middle cerebral artery occlusion（ MCAO）. They were performed by ischemia 2 h and then used for reperfusion. After 6 h,acupuncture group was accepted treatment by electroacupuncture＂top Siguan＂,once a day. The rabbits in the three groups were respectively executed and got cerebral tissue in 1 d,3 d and 7 d. Then they were determined the level of dopamine（ DA）,noradrenaline（ NE） and 5-hydroxytryptamin（ 5-HT）by using fluorospectrophotometry,and detected the content of Na＋-K＋-ATP and Ca2 ＋-ATP by using colorimetry. Results Compared by sham-operated group in the same time,DA,NE and 5-HT were decreased obviously in model group especially in the 1 d group,but DA,NE and 5-HT somewhat increased with cerebral ischemia reperfusion time gone by. Na＋-K＋-ATP and Ca2 ＋-ATP decreased obviously,the differences was statically significant（ P 0. 01）. Compared with the model group in every time,the level of DA,NE and 5-HT,the content of Na＋-K＋-ATP and Ca2 ＋-ATP were increased in different degree（ P 0. 01）.Conclusion Acupuncture ＂Top siguan ＂can improve ATP enzyme metabolism,can reduce monoamine neurotransmitter release and inhibit monoamine neurotransmitter toxicity effect in cerebral ischemia reperfusion rabbit. so needling＂Top siguan＂can play a role of resistance ischemic injury.