摘要
采用单因素试验及正交试验设计,利用乙醇溶剂浸提结合微波辅助提取法对石仙桃(Phoidotaof chinensis Lind L.)总黄酮的提取工艺进行优化,并用分光光度计比色法测定提取的总黄酮含量。结果表明,石仙桃总黄酮提取的最佳工艺条件为微波功率300 W,提取时间30 min,温度60℃,料液比(m∶V,g∶m L)1∶20,乙醇体积分数80%。按照最佳工艺条件进行提取,石仙桃总黄酮提取量达31.41 mg/g。
The single factor and orthogonal test were designed to optimize the Microwave-assisted ethanol extraction method to extract the total flavonoids in Pholidota chinensis lindl, and the total flavonoids content of P. chinensis Lind was detected by ultraviolet-visible absorption spectroscopy.Results showed that the optimal extraction concentration of flavonoids was 80%（V/V） ethanol,extraction for 30 min,solid to liquid ratio at 1：20 （m：V,g：mL）,and extraction temperature at 60℃.Under the optimum conditions,the content of extracted total flavonoids from P. chinensis Lind was 31.41 mg/g.
出处
《湖北农业科学》
2015年第9期2157-2160,共4页
Hubei Agricultural Sciences
基金
江西省自然科学基金项目(2012ZBA20513)
赣南医学院产学研项目(201205)
