响应面法对甘薯茎叶绿原酸超声提取工艺的优化

Optimization of Ultrasonic Extraction of Chlorogenic Acid from Leaves and Stems of Sweet Potato by Response Surface Methodology

为优化甘薯[Ipomoea batatas(L.)Lam.]茎叶中绿原酸超声提取工艺,在单因素试验的基础上,选择乙醇溶液体积分数、提取时间、料液比(g:m L,下同)3个对绿原酸提取率影响较大的因素,采用BoxBehnken中心组合试验设计对各因素进行优化组合,运用Design Expert 8.05b软件进行响应面回归分析,建立了绿原酸提取率的二次多项式回归方程。得到的优化组合条件为乙醇溶液体积分数70%、料液比1∶20、超声时间24 min,绿原酸提取率为3.097 2%,与响应面拟合所得方程的预测值3.106 8%相近。试验所用甘薯茎叶价廉易得,提取方法高效可行,分析方法可信,可为甘薯茎叶的开发利用提供依据。

In order to optimize the ultrasonic extraction technology of chlorogenic acid from leaves and stems of Ipomoea batatas （L.） Lam.,on the base of single-factor experiment,it selected the following three factors which greatly influnence the extraction ratio of chlorogenic acid： the volume fraction of ethanol solution,the extraction time and solid-liquid ratio. Then, it adopted the combination experiment design of Box-Behnken centre to optimize those factors, used Design Expert 8.05b soft- ware to analyze the response surface regression and established quadratic polynomial regression equation of the extraction ratio of chlorogenic acid. The optimum combination condition obtained were the follow spects： the volume fraction of ethanol solu- tion was 70%, solid-liquid ratio was l：20（g：mL）,extraction time was 24 min and the extraction yield of chlorogenic acid was 3.097 2%, which was well consistent with the predicted value of the response surface 3.106 8%. The extraction method has the advantage of time-saving, high efficiency and good reliablity of analysis method,which can provide the basis for the de- velopment and utilization of the leaves and stems of Ipomoea batatas（L.） Lam..