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血清肺炎支原体DNA定量对肺炎支原体感染的诊断价值 被引量:8

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摘要 目的探讨实时荧光定量PCR法诊断肺炎支原体感染的可行性。方法收集肺炎支原体感染患者60例(支原体感染组),健康体检正常的个体34例(健康对照组)。用凝集法检测血清肺炎支原体Ig G抗体,用实时荧光定量PCR法检测血清肺炎支原体DNA,用免疫比浊法检测C反应蛋白,用染色后激光扫描法检测全血白细胞及中性粒细胞。结果与健康对照组相比,治疗前支原体感染组的血清肺炎支原体DNA定量、肺炎支原体Ig G抗体滴度、C反应蛋白浓度、全血白细胞及中性粒细胞计数均显著升高;与治疗前相比,支原体感染组治疗后的血清肺炎支原体DNA定量、全血白细胞及中性粒细胞计数均比治疗前显著下降;与治疗前相比,支原体感染组治疗后的肺炎支原体Ig G抗体滴度显著升高,C反应蛋白浓度差异无统计学意义。结论实时荧光定量PCR法对肺炎支原体感染的诊断与治疗后监测比肺炎支原体抗体血清学检测更优。
出处 《广东医学》 CAS 北大核心 2015年第12期1880-1882,共3页 Guangdong Medical Journal
基金 广东省佛山市医学类科技攻关项目(编号:2014AB001803)
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