复发性流产病人绒毛组织中FK506结合蛋白52的表达及意义

Expression and significance of FK506 binding protein 52 in chorionic villi of patients with recurrent spontaneous abortion

目的探讨复发性流产(RSA)病人及正常早孕妇女绒毛组织中FK506结合蛋白52(FKBP52)基因和蛋白的表达。方法选取2012年2月至2013年6月广西医科大学第一附属医院妇产科门诊就诊的30例RSA妊娠妇女(复发性流产组)及同期正常早孕妇女30例(对照组),收集其新鲜绒毛组织,分别应用实时定量聚合酶链反应(RT-PCR)及免疫组织化学方法检测两组绒毛组织中FKBP52基因和蛋白的表达。结果 FKBP52在两组妇女的绒毛组织中均有表达,但与对照组相比,复发性流产组凝胶成像系统半定量分析FKBP52 m RNA(1.484±0.198对1.109±0.292,P<0.05)及蛋白表达(91.8±5.3对68.2±8.4,P<0.01)均明显降低。结论 FKBP52的表达可能在胚胎着床和早期妊娠的维持中起重要作用,其表达降低可能与RSA的发病密切相关。

Objective To investigate the mRNA and expression of FK506 binding protein 52 （FKBP52） in the chorionic villi of patients with recurrent spontaneous abortion （RSA） and normal women during early pregnancy. Methods Fresh chorionic villus tissues were collected from 30 patients with a history of RSA （experimental group）and 30 normal early pregnant women （control group） at Department of Obstetrics and Gynecology, the First Affiliated Hospital of Guangxi Medical University between February 2012 and June 2013.The FKBP52 mRNA and protein expression levels were detected by semiquantitative reverse transcription polymerase chain reaction and immunohistochemistry, re- spectively. Results FKBP52 was expressed in chorionic villi samples of normal early pregnant women and RSA patients.However, RSA patients exhibited significantly lower FKBP52 mRNA （1.109 ± 0.292 vs.1.484 ±0.198, P〈0.05） and protein （IOD： 68.2 ±8.4 vs.91.8 ± 5.3, P〈0.01 ） expression levels compared with those in normal early pregnancies. Conclusion The expression of FKBP52 may play a key role in embryo implantation and early pregnancy maintenance, the low exprssion of FKBP52 may be involved in the pathogenesis of RSA.