联苯双酯和齐墩果酸对ANIT致小鼠、大鼠肝损伤的作用研究

The Protective Effects of Bifendate and Oleanolic acid on Acute Hepatic Injury Induced by ANIT in Mice and Rat

目的观察联苯双酯和齐墩果酸对异硫氰酸-1-萘酯(α-naphthyl isothiocyanate,ANIT)橄榄油溶液致肝损伤小鼠、大鼠的保护作用。方法先给予小鼠、大鼠联苯双酯和齐墩果酸,然后对小鼠、大鼠分别灌胃给以100 mg·kg-1、80 mg·kg-1ANIT造成肝损伤,48 h后测其空腹血清中的ALT、AST、TBIL、DBIL、GGT、ALP,并剖取肝、脾,称湿重,计算其脏器指数。结果齐墩果酸具有降低小鼠ALT、AST和TB值,联苯双酯反而增加小鼠ALT、AST和TB值。联苯双酯具有降低大鼠ALT、AST和ALP值,齐墩果酸则不降低大鼠ALT和AST值。结论齐墩果酸对ANIT致肝损伤小鼠具有保肝作用,联苯双酯对ANIT致肝损伤大鼠具有保肝作用。

Objective To observe the protective effects of Bifendate and Oleanolic acid on acute hepatic injury induced by ANIT in olive oil in mice. Method At first,Bifendate and Oleanolic acid were taken by mice and rats. And then 100 mg / kg and 80 mg / kg ANIT were injected into abdominal cavity of mice and rats to damage their liver,respectively. After 48 hours,their fasting serum levels of ALT,AST,TBIL,DBIL,GGT,ALP were measured. The animals were sacrificed and dissected,and the wet weight of their liver and spleen as well were measured. Finally the viscera index was calculated. Result Oleanolic acid significantly reduced the serum ALT,AST and TB in mice,but Bifendate had no liver protection capacity. Bifendate significantly reduced the serum ALT,AST and ALP in rat,but Oleanolic acid had no liver protection effect. Conclusion Oleanolic acid has liver protection effect on liver injury induced by ANIT in olive oil in mice,and Bifendate has liver protection effect on liver injury induced by ANIT in olive oil in rat.