青蒿渣中总多糖提取工艺及其抗氧化活性

Optimization of Extraction Process for Total Polysaccharides from Artemisiae Annuae Herba Residue by Response Surface Methodology and Evaluation of Its Antioxidant Activity

目的:优选青蒿渣中总多糖的提取工艺参数并探究其抗氧化能力。方法:以液料比、提取温度、提取时间为自变量,总多糖得率为因变量,利用响应面法优选青蒿渣总多糖的超声提取工艺。通过清除1,1-二苯基-2-苦基肼自由基、清除羟自由基及油脂抗氧化试验,与抗坏血酸的抗氧化能力进行比较,评价青蒿渣总多糖的抗氧化能力。结果:最佳提取工艺条件为液料比30∶1,提取时间30 min,提取温度70℃;青蒿渣总多糖提取率1.773 3%。青蒿渣总多糖的抗氧化能力随质量浓度的增大而增强,其清除羟自由基的能力较抗坏血酸强,两者的半抑制浓度分别为(164.26±0.84),(214.89±0.18)mg·L-1。结论:优选的提取工艺稳定可靠,青蒿渣总多糖具有一定抗氧化能力,为青蒿资源的综合利用提供参考。

Objective： To optimize extraction process of total polysaccharides from Artemisiae Annuae Herba residue and investigate its antioxidant activity. Method： Response surface methodology was employed to optimize extraction process of total polysaccharides from Artemisiae Annuae Herba residue by taking liquid-solid ratio, extracting temperature and time as factors. Antioxidant activity of total polysaccharides was evaluated through several in vitro antioxidant assays, such as 1, 1-diphenyl-2-picrylhydrazyi （DPPH） radical scavenging activity, hydroxyl radical scavenging activity and anti-lipid peroxidation with vitamin C as a reference. Result： Optimum extraction conditions of total polysaccharides were as follows ： liquid-solid ratio of 30：1 , extraction time of 30 rain and extraction temperature of 70 ~C. Under these conditions, yield of total polysaccharides could be up to 1. 773 3%. Antioxidant capacity of total polysaccharides increased with the concentration of total polysaccharides increased, its scavenging ability towards hydroxyl free radical was stronger than vitamin C. Conclusion： Optimized extraction process is stable and reliable, total polysaccharides from Artemisiae Annuae Herba residue has antioxidant capacity, which can provide a reference for comprehensive utilization of Artemisiae Annuae Herba.