贵州地方茶树品种资源遗传多样性RAPD分析

Analysis of Genetic Diversity with RAPD Markers for Local Tea Populations in Guizhou

为培育特色优良品种,采用RAPD分子标记对贵州4个地方茶树品种群体的遗传差异进行分析。结果显示,‘贵定鸟王茶’、‘都匀毛尖茶’、‘湄潭苔茶’和‘石阡苔茶’品种群体内种质间遗传距离的变幅较大,分别为0.1792~0.8328、0.1745~0.8518、0.1480~0.6507、0.1516~0.8691,Nei’s基因多样性与Shannon信息指数及品种内RPAD谱带多态性显示它们的遗传多样性依次降低。4个地方群体品种遗传多样性丰富,遗传变异主要发生在群体内。

The genetic diversity of populations of four local tea varieties [Camellia sinensis（L.） O.Kuntze] inGuizhou was analyzed using random amplified polymorphic DNA（RAPD） markers to breed cultivars withexcellent characteristics.The genetic distance of four populations including C.sinensis ‘Guiding-niaowangcha’（G）,C.sinensis‘Duyun-maojiancha’（D）,C.sinensis‘Meitan-taicha’（M）,and C.sinensis‘Shiqian-taicha’（S） was 0.1792-0.8328,0.1745-0.8518,0.1480-0.6507,and 0.1516-0.8691,respectively.In addition,based on Nei’s gene diversity index,Shannon’s index,and RAPD bands polymorphism analysis,the genetic diversity among four populations was G〉D〉M〉S.The data showed that these populations had arich genetic diversity.Their genetic variation was mainly distributed within populations.