不同黄瓜材料抗蚜性与部分次生代谢物及其相关酶活力的关系

The Relationship Between Aphid-resistance of Different Cucumber Materials and Part of Secondary Metabolites and Their Related Enzyme Activities

为筛选具有高抗蚜性的黄瓜新品种,并为黄瓜抗蚜性鉴定提供理论依据。根据蚜量比值法,以有毛黄瓜为不抗蚜对照,无毛黄瓜为抗蚜对照,对国内外370份黄瓜种质资源进行抗蚜性筛选分级,并对筛选出的不同抗蚜等级的黄瓜材料接种蚜虫处理,研究接种蚜虫前后叶片中黄酮、单宁、总酚含量及苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)活力变化。筛选出抗性材料118号、中抗材料311号和227号、低抗材料321号。其中,高抗材料无毛黄瓜的黄酮含量及PAL、PPO的活力最高;抗性材料118号的单宁、总酚含量最高,PPO活力仅次于无毛黄瓜;中抗材料227号的单宁、总酚含量及PPO活力仅次于118号;中抗材料311号的黄酮、总酚含量及PAL活力较高;低抗材料321号的PAL活力仅次于无毛黄瓜;各抗蚜材料的黄酮含量及PAL和PPO的活力均高于不抗蚜对照有毛黄瓜。由此得出结论:不同黄瓜材料抗蚜性与黄酮、单宁、总酚含量及PAL、PPO活力中的一种或几种物质综合作用有密切关系。

The paper aims to breed new cucumber varieties with high resistance to aphids,and providetheoretical basis for the identification of cucumber aphid-resistance.According to the method of ratio of aphid,the hairy cucumber（an ordinary cucumber） was used as the control of less anti-aphid ability,while theglabrous cucumber（the mutant of the ordinary cucumber,its surface is smooth without hairs,with highanti-aphid ability） was used as the control of anti-aphid,370 home and abroad cucumber germplasm resourceswere taken as test materials to the anti-aphid screening classification,and aphids were inoculated to thecucumber materials with different levels of anti-aphid ability.The author studied the content change of somesecondary metabolites including flavonoids,tannins and total phenol,and the enzyme activities of phenylalnineammonialyase（PAL） and polyphenol oxidase（PPO） in the leaves before and after inoculation of aphids.Theresults showed that： the anti-aphid material was No.118,the medium anti-aphid materials were No.311 and No.227,and the low anti-aphid material was No.321.After inoculating aphids in leaves,the content offlavonoids and the activity of PAL and PPO in the leaves of the glabrous cucumber,which was the high anti-aphid material,were higher than that of the other anti-aphid materials; the contents of tannins and total phenolin the leaves of No.118,which was the anti-aphid material,were the highest among the anti-aphid materials,and the activity of PPO in its leaves was second only to that of the glabrous cucumber; the content of tanninsand total phenol,as well as the activity of PPO in the leaves of the medium anti-aphid material No.227 weresecond only to that of No.118; the content of flavonoids and total phenol,as well as the activity of PAL in theleaves of the medium anti-aphid material No.311 were higher; the activity of PAL in the leaves of the low anti-aphid material No.227 was second only to that of glabrous cucumber; the content of flavonoids,as well as theactivity of PAL and PPO in the leaves of the anti-aphid materials were all higher than that of the hairycucumber.In conclusion,by this test,cucumber materials with different levels of anti-aphid were screened,and aphid-resistance of different cucumber materials had a close relationship with the contents of flavonoid,tannin and total phenol which were secondary metabolites,as well as the PAL and PPO which were the mostimportant enzymes in the secondary metabolic processes.