姜黄素丝素蛋白微球的制备及药物释放研究

Study on Preparation of Curcumin-Loaded Silk Fibroin Microspheres and Drug Release

报道了一种简单制备姜黄素丝素蛋白缓释微球的方法，并通过扫描电子显微镜、荧光显微镜、动态光散射、X射线衍射等手段研究了丝素蛋白载药微球的形成、形态大小、药物包覆及缓释性能。结果表明：制备的姜黄素丝素蛋白微球呈圆球形，粒径在210～320nm，分散指数为0．17左右；随着丝素蛋白质量分数增加，栽药微球粒径增大；而姜黄素初始质量浓度的增大，可使微球粒径略有减小；载药微球的包封率及载药质量分数分别可达40．27％及1．22％；体外药物释放实验显示，姜黄素的释放速率可以通过控制释放介质中乙醇的体积分数进行调节。

This paper reports a simple method to prepare curcumin-loaded silk fibroin microspheres. The formation,morphology, structure, size, drug encapsulation and drug release property of curcumin-loaded silk fibroin microspheres were studied by Scanning Electron Microscopy （SEM）, Fluorescent Microscope, Dynamic Light Scattering measurements （DLS） and X-ray Diffraction （XRD）. It is found that the curcumin-loaded silk fibroin microspheres are globular particles with grain diameter ranging from 210 nm to 320 nm and the PDI is around 0.17. The grain diameter of drug-loaded microspheres increases with the rise in mass fraction of silk fibroin ; the increase in the initial concentration of the curcumin can make grain diameter of microspheres decrease slightly ; the encapsulation efficiency and drug loading mass fraction of drug-loaded microspheres can achieve 40.27% and 1.22%,respectively. The study on in-vitro drug release shows that release rate of curcuinin can be adjusted by controlling volume fraction of ethanol in release medium.