大孔树脂对蛹虫草粗多糖的脱色工艺研究

Study on Decoloration Process for Crude Polysaccharides from Cordyceps militaris Link. by Macroporous Resin

【目的】探讨大孔树脂对蛹虫草粗多糖脱色的最佳工艺参数条件。【方法】以脱色率、多糖保留率为指标,利用静态吸附脱色实验对7种大孔树脂进行优选,并通过静态/动态吸附脱色的单因素影响实验,对筛选树脂脱除蛹虫草粗多糖色素的最佳条件进行考察。【结果】D941大孔树脂的脱色效果最好,其最佳脱色条件为:样品起始浓度为50 mg/m L,溶液p H值为5.5,操作温度为45℃;动态吸附流速为2.5床体积/h(BV/h),样品溶液上样量为5 BV,最优条件下,脱色率和多糖保留率分别为(93.9±3.4)%和(91.7±2.2)%。【结论】D941树脂适合应用于蛹虫草粗多糖的脱色,本研究优化方法可行、可靠。

Objective To study the optimum decoloration technological conditions of crude polysaccharides from Cordyceps militaris Link.（PCM） by macroporous resin. Methods With the decolorization rate and retention rate of PCM as indexes, static adsorption factor experiment was used for the optimization of seven kinds of macroporous resin. And then the optimum conditions of decolorization of PCM were investigated by the static/dynamaic adsorption single-factor experiments. Results D941 macroporous resin is the best. The optimum decoloration technological conditions were as follows： initial concentration of the sample was 50 mg/m L, p H value was 5.5, operating temperature was 45℃, the flow rate of dynamaic adsorption was 2.5 BV/h, and the sample solution volume was 5 bed volume per hour. Under the optimal conditions, decolorization rate and retention rate of PCM were（93.9 ± 3.4） % and（91.7 ± 2.2） %, respectively. Conclusion The resin D941 is suitable for the decoloration of PCM. The optimized method is feasible and reliable, having the prospects for popularizing.