胃肠肿瘤患者外周血PCDHl7mRNA定量检测的临床意义

The clinical significance of quantitative detection of peripheral blood PCDH17mRNA of patients with gastrointestinal cancer

目的分析原钙黏素17（PCDHl7）mRNA在胃肠肿瘤患者外周血中表达的临床价值。方法对48例胃肠肿瘤患者分别采用荧光定量PCR方法检测其外周血原钙黏素17 （PCDHl7） mRNA，采用免疫组化方法检测其肿瘤组织的PCDHl7蛋白。结果胃肠组织中PCDHl7蛋白阳性表达率为58．57％，外周血原钙黏素17阳性率为61．81％。治疗前外周血原钙黏素17RNA，治疗前、后PCDHl7mRNA含量差值的含量在不同性别、年龄、治疗组及病理类型间差异无统计学意义，在有无淋巴结、远处转移及最大肿瘤患者直径组差异有统计学意义，治疗前、后PCDHl7mRNA含量差异的不同疗效无统计学意义。结论胃肠肿瘤患者外周血PCDHl7mRNA监测可以代替瘤组织PCDHl7蛋白的监测。

Objective To analyze the clinical value of PCDH17 mRNA expression in peripheral blood of patients with gas- trointestinal tumor. Methods 48 cases of gastrointestinal cancer patients respectively receiving fluorescence quantitative poly- merase chain reaction to detect peripheral blood PCDH17 mRNA of, using immunohistochemical method to detect the PCDH17 protein in tumor tissue. Results In gastrointestinal tissues PCDH17 protein positive expression rate was 58.57%. Positive rate of PCDH17 in peripheral blood was 61.81%. Before and after treatment, PCDH17mRNA content difference in different genders, ages, groups and pathological patterns had no statistical significance. While in groups with or without lymphonodus, distant metastasis and maximum diameter of tumor patients group, the difference had statistical significance. Before and after treatment, PCDH17mRNA content difference in treatment effect group had no statistical significance. Conclusion Tumor tissue protein PCDH17 detection can be replaced by peripheral blood PCDH17mRNA detection.