β-防御素2与病原疫苗及其免疫效应的关联性研究

Research on interaction between β-defensin 2 and pathogen vaccines

目的研究病原菌疫苗及其免疫反应与大鼠β-防御素2蛋白（rBD-2）表达的关系，探讨β-防御素2人为诱导的方法及其在疫苗免疫防御中的作用。方法使用两种疫苗（23价肺炎链球菌多糖疫苗、B型流感嗜血杆菌结合疫苗）分别滴人大鼠肺内，Western blotting法检测肺组织rBD-2蛋白含量；选择作用显著的疫苗滴人鼠肺，不同时间检测肺组织rBD-2蛋白含量，EUSA检测血清中TNF-α、IL—1β、和IL-10蛋白含量。结果两组疫苗作用大鼠后肺组织中rBD-2蛋白均明显上升，B型流感嗜血杆菌结合疫苗的作用更明显；B型流感嗜血杆菌结合疫苗作用后21d，rBD-2随时间逐渐升高，血清细胞因子含量均呈先升高、后降低的趋势，与对照组比较差异显著。结论病原菌疫苗可明显上调大鼠肺组织rBD-2的表达，rBD-2与疫苗引起的免疫反应存在关联，在疫苗的免疫防御效应中有一定作用。

Objective To study the relationship between the pathogenic bacteria vaccine and its immune response with expression of β- defensin-2 （rBD-2） in rats and investigate the method of artificially induced β-defensin-2 and its role in the immune defense of the vaccine. Methods Two vaccines （23-valent pneumococcal polysaccharide vaccine and Haemophilus influenzae type B conjugate vaccine） were trickled into rat lungs, respectively. Western blotting was used to detect the concentration of rBD-2 protein in the lungs tissues. The more effective vaccines was selected to trickle into the rat lung. The rBD-2 protein in the lung tissue was evaluated by Western blotting and the levels of serum TNF-α, IL-1β, and IL-10 were measured by ELISA at different times. Results The level of rBD-2 protein in the rat lung tissue was significantly increased after two vaccines treatment. The effect of Haemophilus influenzae type B conjugate vaccine was more obvious than 23-valent pneumococcal polysaccharide vaccine. After treatment with Haemophilus influenzae type B conjugate vaccine for 21 days, compared with control group, the level of rBD-2 protein was gradually increased with time, and the levels of serum cytokines showed significant increase firstly, then significant decrease. Conclusion Pathogen vaccine significantly upregulates expression of rBD-2 in rat lung, and rBD-2 associates with the vaccine-induced immune responses and plays a role in the immune defense of vaccine.