棘阿米巴角膜炎患者分离株在不同环境中的形态观察

Morphology observations of Acanthamcoba spp. Isolates from patients with Acanthamoeba keratitis in different environments

目的观察棘阿米巴的形态学特点,为探讨棘阿米巴感染的诊断提供依据。方法以棘阿米巴角膜炎患者的送检材料为实验材料,置入不同条件中培养,观察其在不同的p H值（2、4、6、8、10）、温度（20、25、30、35℃）和加大肠埃希菌的条件下对棘阿米巴形态和活力的影响。结果棘阿米巴在不同的条件下形态呈多样性,酸性环境中以滋养体为主,而在碱性环境易形成包囊。p H=6,温度为25~30℃和加有大肠埃希菌的培养条件时,适合棘阿米巴生长,其中大部分的虫体为活动滋养体,并进行大量的繁殖。结论棘阿米巴在不同条件中存在滋养体和包囊转化过程,这为进一步研究其诊断及致病机制奠定了基础。

Objective To observe the morphological characteristics Acanthamoeba, and provide theoretical basis for diagnosis of Acanthamoeba infection. Methods specimens from Acanthamoeba keratitis patients was cultured in different conditions, and observed the morphology and viability of Acanthamoeba in different culture p H（2, 4, 6, 8, 10）, temperature（20,25, 30, 35℃）, and with and without addition of E. coli bacteria. Results Acanthamoeba trophozoites existed mainly in the acidic environment and form cysts in the alkaline environment. And Acanthamoeba were morphologically diverse under different conditions. Acanthamoeba grew the best under the culture environment of p H6, 25~30℃ with E. coli bacteria, and most of the Acanthamoeba is active trophozoites and multiply through division. Conclusion The morphology observations of transformation process between Acanthamoeba trophozoites and cysts, which contributes to clinical diagnosis and pathogenic mechanisms of Acanthamoeba.