MCP-1基因多态性与结核性脓胸发病风险的关系

Association of MCP-1 gene polymorphism to the risk of tuberculous empyema

目的探讨中国河北地区MCP-1(单核细胞趋化因子蛋白-1)基因-2518A/G和-362G/C单核苷酸多态性(SNP)与结核性脓胸的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法对结核性脓胸患者500例和健康对照组500例进行MCP-1基因-2518A/G和-362G/C SNPs检测。结果与MCP-1-2518A/A基因型相比,携带A/G和G/G基因型均可增加结核性脓胸发病风险(OR=2.254和OR=8.728)。进行分层分析发现,无卡介苗(BCG)接种史、体质量指数(BMI)<18.5均增加结核性脓胸发病风险(OR=3.309和OR=2.767)。MCP-1-362G/C SNP无统计学意义。结论 (1)MCP-1基因-2518A/G SNP可能与结核性脓胸的发病风险有关。(2)-362G/C SNP可能与结核性脓胸无关。

Objective To study the association of the single nucleotide polymorphism of MCP-1 -2518A/ G and -362G/ C to the risk of tuberculous empyema （TE）. Methods The SNPs of MCP-1 -2518A/ G and -362G/ C were analyzed by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） in 500 patients and 500 healthy control individuals. Results Compared with the MCP-1-2518 A/ A genotype, the A/ G and G/ G genotypes could significantly increase the risk of developing TE （OR = 2. 254 and 8. 728）. When stratified for history of BCG vaccination and BMI status, the individuals, especially in the group without history of BCG vaccination or in the BMI 〈 18. 5 group, were associated with the increased risk of developing TE （OR = 3. 309 and 2. 767）. There was no significant association of the MCP-1-362G/ C SNP to the risk of developing TE. Conclusion The MCP-1-2518A/ G SNP is associated with susceptibility to tuberculous empyema. The-362G/ C SNP might not be related to the risk of TE.