具有拮抗作用的石榴内生菌的分离与鉴定

Isolation and Identification of Endophytic Bacteria in Pomegranate with Antipathogenic Functions

[目的]筛选对石榴枯萎病菌具有拮抗作用的内生生防菌。[方法]从采自云南蒙自的石榴叶片中分离并获得5株内生拮抗菌,通过测定其对甘薯长喙壳(Ceratocystis fimbriata)的抑菌能力筛选出1株具有高效拮抗作用的生防菌株Y2,对其进行抑菌谱测定及鉴定。[结果]经生理生化和16S r DNA序列测定表明Y2为枯草芽孢杆菌(Bacillus subtilis)。[结论]试验结果为石榴枯萎病的防治提供了理论依据。

[ Objective] The aim was to screen out endophytic bacteria from pomegranate with antipathogenic functions. [ Method] Five strains endophytic bacteria were isolated from leaves of pomegranate collected in Mengzi County of Yunnan Province and its inhibition was identified. One strain Y2 having good inhabit effect was selected through tested the inhabit effect for Ceratocystis fimbriata, and tested the antibacterial spectrum. [ Result] According to physiological and biochemical as well as 16S rDNA sequencing, the strain Y2 belonged to Bacillus subtilis. [ Conclusion] The results provide theoretical basis for the control of pomegranate wilt disease.