微柱凝胶卡式法和试管抗人球蛋白法在新生儿ABO溶血三项试验中的效果观察

Effect observation of micro-column gel cards assay and anti-human globulin test in tube on three tests of neonatal ABO hemolysis

目的 比较微柱凝胶卡式法和试管抗人球蛋白法在新生儿ABO溶血三项试验中的效果。方法 选取本院2013年1月-2014年12月收治的临床拟诊的108例ABO母婴血型不合新生儿溶血病患者作为研究对象,分别采用微柱凝胶卡式法和传统试管法进行直接抗人球蛋白试验、间接抗人球蛋白试验、放散试验,并对检测结果进行对比分析。结果 108例患者中,确诊102例,其中微柱凝胶卡式法所测得的抗体游离试验阳性率、直接抗人球蛋白试验阳性率、抗体放散试验阳性率、符合ABO-HDN者所占比例显著高于传统试管法,差异有统计学意义（P〈0.05）。结论 微柱凝胶卡式法在新生儿溶血病诊断中的应用价值显著,且操作简单,耗时较短,值得临床推广应用。

Objective To compare the effect of micro-column gel cards assay and anti-human globulin test in tube on three tests of neonatal ABO hemolysis. Methods 108 cases with neonatal hemolysis due to ABO blood group incompatibility of mom infant from January 2013 to December 2014 in our hospital were selected as research object.The direct anti-human globulin test,indirect anti-human globulin test and elution test were carried out by micro-column gel cards assay and traditional tubing,the tested outcomes was compared and analyzed. Results Among 108 cases,102 cases were definitely diagnosed.The positive rate of antibody free test,direct anti-human globulin test,antibody elution test and the proportion of patients meeting the diagnosis of ABO-HDN by micro-column gel cards assay was higher than that by the traditional tubing method,with significant difference（P〈0.05）. Conclusion Application vaule of microcolumn gel card method in the diagnosis of hemolytic disease of newborn is significant,and the operation is simple,time is short,it is worthy of clinical promotion and application.