调节性T细胞在冠心病与牙周炎相互关系中的作用

Role of regulatory T cell in relationship between coronary heart disease and periodontitis

目的探讨调节性T细胞在牙周炎促进冠心病进展中所起的作用。方法将90例患者依据冠状动脉造影结果及牙周炎Armitage诊断标准分为:胸痛综合征组(CPS组)、冠状动脉粥样硬化性心脏病(冠心病)组(CHD组)和冠心病合并牙周炎组(CHD-P组);再将CHD-P组分为冠心病合并轻、中度牙周炎组(CHD-P1组)、冠心病合并重度牙周炎组(CHD-P2组)。采用流式细胞仪分析CD4+CD25+Foxp3+Treg细胞占T淋巴细胞的比例。结果 Gensini评分CHD-P2组(65.86±39.94)较CHD-P1组(31.23±33.53)和CHD组(13.09±7.59)明显升高,差异有统计学意义(P<0.05)。CHD-P1组较CHD组Gensini评分明显升高,差异有统计学意义(P<0.05)。CD4+CD25+Foxp3+Treg/CD4+T在CHD-P组为[(1.93±0.46)%]较CHD组[(2.32±0.72)%]和CPS组[(2.54±0.95)%]明显降低,差异有统计学意义(P<0.05)。结论牙周炎使冠心病患者的调节性T细胞数量进一步下降,调节性T细胞与牙周炎促进冠心病的进展密切相关。

Objective To investigate the role of regulatory T cells in progress of coronary heart disease （CHD） promoted by periodontitis. Methods CHD patients （n=90） were divided, according to outcomes of coronary angiography （CAG） and Armitage diagnostic criteria of periodontitis, into chest pain syndrome group （CPS group, CHD group and CHD combining periodontitis group （CHD-P group）, and then CHD-P group was further divided into CHD-P1 group （CHD combining mild-moderate periodontitis） and CHD-P2 group （CHD combining severe periodontitis）. The proportion of CD4＋CD25＋Foxp3＋Treg cells in regulatory T cells was analyzed by using flow cytometry. Results Gensini scores increased significantly in CHD-P2 group （65.86±39.94） compared with CHD-P1 group （31.23±33.53） and CHD group （13.09±7.59, P〈0.05）. Gensini scores increased significantly in CHD-P1 group compared with CHD group （P〈0.05）. CD4＋CD25＋Foxp3＋Treg/CD4＋T decreased in CHD-P group [（1.93±0.46）%] compared with CHD group [（2.32±0.72）%] and CPS group [（2.54±0.95）%, P〈0.05]. Conclusion The quantity of regulatory T cells can be reduced further by periodontitis, so regulatory T cells is closely correlated to progress of CHD promoted by periodontitis.