层流手术室空气中浮游菌超标原因与控制

The causes and control of excessive planktonic bacteria in the air of laminar flow operation room

目的研究层流手术室空气中浮游菌超标的原因与控制,以降低感染发生率。方法对2012年7月-2014年7月层流手术室连续100台手术进行检测,使用浮游菌采样器和尘埃粒子计数仪在手术室中不同的时间段进行多点采样,包括手术区和非手术区(周边区),对所测浮游菌及尘埃粒子进行计数统计,分析浮游菌、尘埃粒子超标的原因,提出处理对策。结果层流手术室在手术前浮游菌、尘埃粒子数量均符合标准,手术开始后浮游菌及尘埃粒子开始呈上升趋势,直到手术结束时达到最大值,在手术结束后浮游菌及尘埃粒子开始下降,30min后恢复标准,差异有统计学意义(P<0.05)。结论在手术室中,尘埃粒子及浮游菌超标的原因是由于手术的动态运行、人员的走动、操作等均导致其上升,而严格的消毒、防止感染是保证手术室洁净的关键。

OBJECTIVE To study the causes and control of excessive planktonic bacteria in the air of laminar flow operation room so as to reduce infection rates .METHODS A total of 100 consecutive operation of laminar flow op‐eration from Jul .2012 to Jul .2014 were detected .Multi‐point sampling in different time period in operation rooms was conducted by planktonic bacteria sampler and dust particle calculating instrument in different areas ,including the operation area and non operation area （peripheral regions） .The planktonic bacteria and dust particle were counted and the causes for excessive planktonic bacteria and dust particle were analyzed to come up with some measures .RESULTS Before surgeries ,numbers of planktonic bacteria and dust particles in laminar flow operation room met the standards .But w hen the operation began ,numbers of planktonic bacteria and dust particle increased rapidly and reached the maximum at the end of the surgery .After that ,the numbers started to reduce and 30 mi‐nutes later regained to the standard .The difference had significance （P〈0 .05） .CONCLUSION In the operation room ,the causes for excessive number of dust particles and planktonic bacteria are dynamic operation ,personnel walking ,and surgery operations .The key point to keep a clean operation room is to disinfect strictly and take pre‐ventive measures .