纳米银比色法检测多巴胺

Colorimetric Detection of Dopamine Based on Silver Nanoparticles

在银纳米粒子存在下,多巴胺可还原硝酸银生成银,导致银纳米粒子粒径增大,从而使溶液颜色发生改变.基于此,提出了一种用于检测多巴胺的纳米银比色法.随着多巴胺浓度的增大,溶液的颜色由浅黄色逐渐变为深黄色,银纳米粒子溶液的吸收峰发生红移且吸光度增大.在最优实验条件下,该方法检测多巴胺的线性范围为0.05~16μmol/L,检出限为0.04μmol/L.该方法操作简单、灵敏且选择性良好,可用于人血清中多巴胺的检测.

A simple and sensitive colorimetric method for the detection of dopamine was proposed based on the redox reaction between silver ion and dopamine, which was catalyzed by silver nanoparticles （AgNPs）, causing the color change. The increase of dopamine concentration led to red shift of absorption peak and increase of the absorbance. Meanwhile, the color of the solution changed from pale yellow and deep yellow Under optimal conditions, a linear range of 0. 05-16 μmol/L between the absorbance and dopamine concentration and a detection limit of 0. 04 μmol/L was obtained. The proposed method was sensitive, simple, lowcost and selective, which could be applied for the detection of dopamine in human serum.