摘要
目的探讨变异型分化抗原簇44(17)(CD44v17)对人宫颈癌HeLa细胞、SiHa细胞系增殖的影响。方法用lipofectamine 2000将CD44v17siRNA、pc DNA3.1-CD44v17质粒及其阴性对照组转染HeLa细胞、SiHa细胞,通过CCK-8、划痕实验、流式细胞术、免疫印迹法(Western blotting)、细胞周期测定方法等观察CD44v17对人宫颈癌细胞系HeLa细胞及SiHa细胞的增殖、凋亡、迁移等能力的影响。结果 CCK-8法证实CD44v17促进宫颈癌细胞的生长;划痕实验显示CD44v17可增强宫颈癌细胞的迁移能力;流式细胞术及Western blotting检测凋亡相关蛋白验证了CD44v17具有抑制宫颈癌细胞凋亡的作用;细胞周期测定显示干扰CD44v17 RNA后,S期细胞比例降低,G1期细胞增高,细胞阻滞于G1期并诱导细胞凋亡。结论 CD44v17能抑制宫颈癌细胞凋亡,促进宫颈癌细胞的生长,增强宫颈癌细胞的增殖迁移能力,它有可能成为宫颈癌治疗的新靶点。
Objective：To explore the effect of CD44v17 on proliferation of human cervical cancer Si Ha cells and HeLa cells.Methods：Lipofectamine 2000 was used to transfect CD44v17 siRNA,pc DNA3.1-CD44v17 plasmid and negative controls into Si Ha cells and HeLa cells; CCK-8 method,wound healing test,flow cytometry,Western blotting,cell cycle analysis were used to observe the effect of CD44v17 on proliferation,apoptosis,and migration of human cervical cancer Si Ha cells and HeLa cells.Results：CCK-8 method confirmed that CD44v17 could promote proliferation of human cervical cancer Si Ha cells and HeLa cells; wound healing test showed that CD44v17 could enhance migration of human cervical cancer Si Ha cells and HeLa cells; flow cytometry and Western blotting verified the inhibiting effect of CD44v17 on apoptosis of human cervical cancer Si Ha cells and HeLa cells; cell cycle analysis showed that after interfering CD44v17 RNA,the proportion of cells at S phase decreased,while the proportion of cells at G1 phase increased,the cells were arrested at G1 phase and cell apoptosis was induced.Conclusion：CD44v17 can inhibit apoptosis,promote proliferation,enhance cell migration of human cervical cancer Si Ha cells and HeLa cells; CD44v17 may be a new target for treatment of cervical cancer.
出处
《中国妇幼保健》
CAS
2015年第20期3486-3490,共5页
Maternal and Child Health Care of China
