不同产地黄芪HPLC/DAD指纹图谱及主要黄酮成分含量测定

HPLC/DAD fingerprints and determination of main flavones in Radix Astragali from different origins

目的:建立黄芪HPLC指纹图谱并测定黄酮类成分的含量,为黄芪药材标准化种植、谱效关系研究及药材质量标准全面提升提供数据支撑。方法:采用HPLC/DAD法建立12批次黄芪药材指纹图谱,并对毛蕊异黄铜苷、芒柄花黄素进行含量测定,色谱柱:Welchrom-C18色谱柱(4.6 mm×250 mm,5μm);梯度洗脱流动相为甲醇-乙睛-0.02 mol·L-1磷酸二氢钠溶液,流速1.2 ml·min-1;检测波长254 nm。结果:应用指纹图谱相似度评价软件找出15个共有峰,指认2个色谱峰,9个样品相似度都在0.900以上,毛蕊异黄酮苷和芒柄花黄素进行定量检测,检测范围内线性关系良好。结论:本研究建立黄芪药材指纹谱方法稳定,数据可靠,可用于控制黄芪药材质量。

OBJECTIVE To establish HPLC fingerprints and determine main flavones in Radix Astragali from different origins,to provide data supports for standardized cultivation,spectrum effect relationship,and comprehensive improvement of quality standards for Radix Astragali.METHODS Fingerprints of Radix Astragali of 12 batches from different origins were obtained by using HPLC/DAD method.Contents of campanulin and formononetin were determined by HPLC/DAD method.Chromatographic separation was performed on C18column（4.6 mm×250 mm,5μm）,with gradient elution of methanol,acetonitrile and 0.02 mol·L-1 monosodium orthophosphate（pH3.2）at a flow rate of 1.2 ml·min-1 at 254 nm.RESULTS Fifteen common peaks were affirmed by＂Chinese traditional medicine chromatographic fingerprint similarity evaluation software＂and 4 peaks were identified.The similarity of 9 batches of samples were more than0.9.Campanulin and formononetin showed good linearity（r0.999 6）within test range.CONCLUSION This method is stable and reliable,which can be used for quality control in production of Radix Astragali.