独山瓜馥木提取物促休眠型结核杆菌复苏的活性研究（英文）

Resuscitating Activity of Fissistigma cavaleriei Extract on Dormant Mycobacteria

结核杆菌具有休眠的特性,休眠型结核杆菌对现有抗痨药物均不敏感,因此研发抗休眠型结核杆菌药物对根治结核病具有重要意义。本试验采用Wayne模型,发现独山瓜馥木根的乙醇提取物能复苏休眠型结核杆菌,独山瓜馥木的促复苏作用可能与刺激休眠型结核杆菌利用C10化合物作为能量来源有关。在体外试验时,独山瓜馥木根的乙醇提取物(2 mg/m L)与异烟肼(8μg/m L)联合用药,可杀死休眠型结核杆菌。结果提示,传统抗痨药物与促进休眠型结核杆菌复苏的药物联合用药可能是根治结核病的一种新策略。

Mycobacterium tuberculosis could go into nonreplicative dormancy.This stage of mycobacteria posed a significant problem for effective therapy as these persistent bacilli were resistant to most of the currently available drugs for the treatment of tuberculosis.The development of drugs that specifically target dormant bacilli could have a profound impact on tuberculosis therapy.In the present study,the extract of Fissistigma cavaleriei root showed a potential activity to resuscitate dormant tubercle bacillus into active bacilli based on Wayne model.It seemed that F.cavaleriei extract might stimulate dormant M.tuberculosis to use compounds of C10 as energy sources for resuscitation.The combination of F.cavaleriei extract （2 mg/mL） and isoniazid （8 μg/mL） showed powerful killing effect on dormant M.tuberculosis. The findings of this study suggested that conventional anti-mycobacterials in combination with ‘dormancy-regrowth’ drugs might be considered as a novel strategy to eradicate latent M.tuberculosis.