忽地笑中石蒜碱酶法提取及分离工艺研究

Study on Enzyme-assisted Extraction and Separation of Lycorine from Lycoris aurea

对复合酶法提取忽地笑石蒜碱的工艺进行优化,并用阳离子交换树脂分离石蒜碱。以纤维素酶与果胶酶的水溶液为提取溶剂,采用L9(34)正交试验考察了酶解p H、酶加入量、酶解时间和酶解温度等影响因素,以石蒜碱得率为指标,得最优提取工艺为:料液比1∶10,p H 4.5,酶添加量4%,酶解温度50℃,提取时间2.0 h,石蒜碱得率为0.1750%。D-001树脂纯化条件为:上样液p H为2,以3 BV/h流速上样,以含1.5 mol/L氨水的70%乙醇洗脱,流速为3 BV/h,初步分离后石蒜碱含量为15.28%。研究结果可为石蒜碱工业化生产提供参考。

The conditions for complex enzymatic extraction of lycorine from Lycoris aurea were optimized by L9（3^4） orthogonal array design.Lycorine was further separated using cation exchange resin.Celluase and pectinase solution was used as the extraction solvent.The effects of pH value of enzyme,amount of complex enzyme,dissociation time and enzymatic hydrolysis temperature on the extraction results were investigated.The optimal conditions were obtained as follows：ratio of solid to liquid was 1：10,pH value was 4.5,amount of complex enzymes was 4%,enzymatic hydrolysis temperature was 50 ℃ and reaction time was 2.0 h.Under these conditions,the extraction yield of lycorine was 0.1750%.In addition,D-001 cation exchange resin was selected for the separation of lycorine.The separation conditions were obtained as follows：adsorption flow rate was 3 BV/h with reagent of pH 2 and the desorption flow rate was 3 BV/h with 70% ethanol solution containing 1.5 mol/L ammonia.After separation,the content of lycorine was increased to 15.28%.The results can provide a reference for industrial production of lycorine.