Au@Pt纳米材料作为模拟酶标记酶联免疫测定克伦特罗

Au@ Pt nimic enzyme labeled ELISA for the detection of clenbuterd

基于Au@Pt纳米材料模拟催化，建立了竞争酶联免疫测定克伦特罗的新方法。优化了克伦特罗抗原与Au@Pt纳米材料偶联条件及Au@Pt标记酶联免疫检测体系的反应温度，H，0：用量等关键参数。在优化条件下，建立的Au@Pt标记酶联免疫检测体系的线性范围为0．0～2．0ng／mL，线性相关系数为0．9912，检测限为0．1ng／mL，低于常规辣根过氧化酶标记酶联免疫吸附试剂盒检测限。在羊尿液样品中添加不同浓度的克伦特罗的回收率高于77．5％。方法用于实际饲喂的羊尿样的检测，其检测结果与液相色谱一串联质谱测定结果能够较好吻合。

A novel competitive enzyme linked immuno sorbent assay （ELISA） for detection of clenbuterol based on Au@ Pt mimic enzyme catalysis has been developed. In this work, we optimized the crucial parameters including conjugation condition of Au@ Pt and antigen, incubation temperature and amount of H202. Under the optimal conditions, the linear range of the developed method was from O. 0 ng/mL to 2. 0 ng/mL and the linearity correlation coefficient was 0. 9912. The limit of detection could be lowered down to 0. 1 ng/mL, lower than commercial HRP enzyme linked immuno sorbent assay, and the recovery of clenbuterol in spiked sheep urine was above 77.5%. Especially, the application of this analytical method was furthur extended to the real sheep urine samples, outputting the results in good accordance with that obtained by liquid chromatography-tandem mass spectrometry.