液质联用法同时测定茶多酚中17种多酚物质

Simultaneous determination of seventeen polyphenols in tea extract by HPLC-MS

建立了同时测定茶多酚中儿茶素类（儿茶素、表儿茶素、表没食子儿茶素、表儿茶素没食子酸酯、表没食子儿茶素没食子酸酯）、黄酮类（山奈酚、杨梅素、芹菜素、槲皮苷、畀槲皮苷）、花青素类（原花青素）、酚酸类（鞣花酸、绿原酸、没食子酸）4大类多酚物质的液质联用检测方法。色谱柱采用Waters Acquity-BEHC18（100mm×2．1mm，1．7μm）；流动相为乙腈和0．1％甲酸，乙腈梯度洗脱浓度和时间为：7％（0min）-7％（3min）-16％（10min）-20％（15min）-30％（16min）-40％（20min）-60％（23min）-100％（24min）；流速：0．3mL／min；柱温：45℃；二极管阵列检测器（PDA）检测波长280nm；进样量1μL。在浓度范围内各组分的浓度和峰面积之间有良好的线性关系，R2〉0．9983；各组分回收率在99．0％～100．43之间。

In this study, a novel method for simultaneous determination of the main components of tea polyphenols, such as catechins （catechin, epicatechin, epigallocatechin, epigallocatechin gallate andepigallocatechin gallate ）, flavonoids （kaempferol, myricetin, apigenin, quercitrin and quercetin- 3 - glucoside） , anthocyanins （proanthocyanidin） and phenoic acids （ellagic acid, chlorogenic acid and gallic acid） , was established by HPLC - MS. Waters Acquity BEH C18 （ 100 mm00rs nm, 1.7 μm） was employed as the chromatographic column, and acetonitrile and 0. 1% formic acid were used as Mobile phase A and B respectively. The programmed concentration of mobile phase A for gradient elution was set as below： 7% （0 min） -7% （3 rain） -16% （10 rain） -20% （15 rain） -30% （16 min） -40% （20 rain） -60% （23 min） -100% （24 min）. Other parameters were fixed as follows： flow rate： 0. 3 mL/min; column temperature： 45℃ ; injection volume： 1μL; PDA detector wavelength： 280 nm. The results showed that the concentrations of each component correlated well with the peak areas （R2 〉0. 9983 ） , and the recoveries were between 98.99% and 100.43%.