摘要
建立了同时测定茶多酚中儿茶素类(儿茶素、表儿茶素、表没食子儿茶素、表儿茶素没食子酸酯、表没食子儿茶素没食子酸酯)、黄酮类(山奈酚、杨梅素、芹菜素、槲皮苷、畀槲皮苷)、花青素类(原花青素)、酚酸类(鞣花酸、绿原酸、没食子酸)4大类多酚物质的液质联用检测方法。色谱柱采用Waters Acquity-BEHC18(100mm×2.1mm,1.7μm);流动相为乙腈和0.1%甲酸,乙腈梯度洗脱浓度和时间为:7%(0min)-7%(3min)-16%(10min)-20%(15min)-30%(16min)-40%(20min)-60%(23min)-100%(24min);流速:0.3mL/min;柱温:45℃;二极管阵列检测器(PDA)检测波长280nm;进样量1μL。在浓度范围内各组分的浓度和峰面积之间有良好的线性关系,R2〉0.9983;各组分回收率在99.0%~100.43之间。
In this study, a novel method for simultaneous determination of the main components of tea polyphenols, such as catechins (catechin, epicatechin, epigallocatechin, epigallocatechin gallate andepigallocatechin gallate ), flavonoids (kaempferol, myricetin, apigenin, quercitrin and quercetin- 3 - glucoside) , anthocyanins (proanthocyanidin) and phenoic acids (ellagic acid, chlorogenic acid and gallic acid) , was established by HPLC - MS. Waters Acquity BEH C18 ( 100 mm00rs nm, 1.7 μm) was employed as the chromatographic column, and acetonitrile and 0. 1% formic acid were used as Mobile phase A and B respectively. The programmed concentration of mobile phase A for gradient elution was set as below: 7% (0 min) -7% (3 rain) -16% (10 rain) -20% (15 rain) -30% (16 min) -40% (20 rain) -60% (23 min) -100% (24 min). Other parameters were fixed as follows: flow rate: 0. 3 mL/min; column temperature: 45℃ ; injection volume: 1μL; PDA detector wavelength: 280 nm. The results showed that the concentrations of each component correlated well with the peak areas (R2 〉0. 9983 ) , and the recoveries were between 98.99% and 100.43%.
出处
《分析试验室》
CAS
CSCD
北大核心
2015年第7期822-826,共5页
Chinese Journal of Analysis Laboratory
关键词
液质联用
测定
茶多酚
HPLC-MS
Determination
Tea polyphenols