碱溶液提取竹叶总黄酮工艺的初步研究

The Pilot Study of Bamboo Leaves Flavonoids Extraction by Alkaline Solution

竹叶黄酮具有较高的药用和食用价值。黄酮类化合物,呈弱酸性,在酸性水溶液难溶,而易溶于碱性水溶液,因此本文考虑用碱性水溶液来提取竹叶的总黄酮。本文采用不同浓度氢氧化钠和碳酸盐水溶液对慈竹叶中总黄酮类成分进行提取,通过分光光度法测定了提取液中的总黄酮含量。实验结果显示,弱碱性的碳酸盐水溶液,更适合用来提取竹叶黄酮。

Bamboo leaves flavonoids have high medicinal and edible value. Flavonoids are weakly acidic, and insoluble in acidic solution and soluble in alkaline solution, so it can be extracted by alkaline solution. In this experiment alkaline solution including different concentration of sodium hydroxide and carbonate aqueous solution, is used to extract flavonoids. Using a spectrophotometer the absorbance of total flavonoids in the extract were measured, and then the concentration of flavonoids could be get. The result shows carbonate aqueous solution is better for bamboo leaves flavonoids extraction.