参苓白术散加减方对结肠黏膜组织水通道蛋白4、水通道蛋白8表达的影响

The effects of modification of prescription of Shenling Baizhu Power on the expressions of AQP4 and AQP8 in colonic mucosa tissue

目的观察参苓白术散加减方对脾虚泄泻模型大鼠结肠黏膜组织水通道蛋白(aquaporin,AQP)4、AQP8的表达的影响。方法健康雄性Wistar大鼠42只随机分为正常组、模型组、高中低剂量组及西药组6组,采用水环境小平台站立法复合高乳糖饲料喂养的方法复制腹泻模型,模型成功后,灌胃给药7天,取结肠组织,通过免疫组化的方法观察结肠黏膜组织AQP4、AQP8的变化。结果与正常组比较,模型组大鼠结肠黏膜组织AQP4、AQP8的表达明显降低;与模型组比较,高中低剂量组及西药组均可以提高结肠黏膜组织中AQP4的表达,高低剂量组和西药组可提高AQP8的表达,中剂量组改善不明显。结论参苓白术散加减方可提高脾虚泄泻模型大鼠结肠黏膜组织中的AQP4、AQP8的表达水平,促进水分的吸收,从而达到止泻的目的。

Objective To investigate the effects ofmodification of prescription of Shenling Baizhu Power on the Expressions of AQP4 and AQP8 proteins in colonic mucosa tissue. Methods Three week-old healthy male rats were randomly dividedinto six groups： control group,model group,high dose group,middle dose group,low dose group and west medicine group. The rats in the model group and treatment groups were given a high lactose diet,and were forced to stand on the multiple platforms which is full of water around to establish the type of spleen deficiency diarrhea. The medicine was given once a day for 7days.And then,AQP4 and AQP8 expression changes in the colon mucosa tissue were observed through the method of immunohistochemistry. Results The expressions of AQP4 and AQP8 in the model group significantlydecreasedcompared to that of in the control group. While compared to the model group,the expression of AQP4 in all the treatment groups were significantly increased,and the expression of AQP8 in the high and low dose groups and west medicine group were significantly increased. Conclusion The modification of prescription of Shenling Baizhu Power can antidiarrhea may via the way of increasing the expressions of AQP4 and AQP8 proteins to adjust the water absorption in colonic mucosa tissue of the rats with spleen deficiency diarrhea.