甘石青黛膏对大鼠亚急性湿疹模型皮损淋巴细胞和角质形成细胞Bcl-2与Bax蛋白表达变化的影响

Effects on the Bcl-2 and Bax protein expression changes of lymphocytes and keratinocyte cells from subacute eczema lesions in rats intervened by Ganshi Qingdai Cream

目的观察甘石青黛膏对大鼠亚急性湿疹模型皮损淋巴细胞和角质形成细胞B淋巴细胞瘤-2(B-cell lymphoma-2,Bcl-2)蛋白与Bax蛋白表达变化的影响,探究中药外治湿疹的分子生物学依据。方法造模前取大鼠正常皮肤组织,制作成石蜡切片备用,造模成功后分为治疗组和模型组,治疗组采用中药甘石青黛膏干预,模型组无干预,检测三组大鼠Bcl-2与Bax蛋白表达情况。结果造模前大鼠Bcl-2与Bax蛋白表达均呈阳性,且Bcl-2/Bax比率接近于1;治疗组Bcl-2蛋白表达呈弱阳性,模型组Bcl-2蛋白表达呈强阳性,两者相比具有显著统计学差异(P<0.001);治疗组Bax蛋白表达呈阳性,模型组Bax蛋白表达呈弱阳性,两者相比具有显著统计学差异(P<0.001);治疗组与模型组Bcl-2/Bax比率相比具有显著统计学差异(P<0.001),后者明显高于前者;治疗组与健康组Bcl-2/Bax比率相比无统计学差(P>0.05)。结论甘石青黛膏具有抑制Bcl-2蛋白表达,促进Bax蛋白表达,使细胞凋亡调控系统趋于平衡的作用。

Objective To observe the effects on the Bcl-2 and Bax protein expression changes of lymphocytes and keratinocyte cells from subacute eczema lesions in rats intervened by Ganshi Qingdai Cream,and to explore the molecular biology basis of traditional Chinese medicine external treatment of eczema. Methods Removing the normal skin tissue in rats before modeling,and making them into paraffin sections to use. After the success of modeling all the rats are divided into the treatment group and the model group,the treatment group is used by traditional Chinese medicine Ganshi Qingdai Cream to intervene,the model group is without intervention. Detecting the Bcl-2 and Bax protein expression in three groups of rats.Results The Bcl-2 and Bax protein expression of rats are positive before modeling,and the Bcl-2 / Bax ratio closes to 1. In treatment group,the Bcl-2 protein expression is weakly positive,and in model group,the Bcl-2 protein expression is strongly positive,there is statistically significant difference between the two groups（ P 0. 001）. In treatment group,the Bax protein expression is positive,and in model group,the Baxprotein expression is weakly positive,the two groups compare with statistically significant difference（ P 0. 001）. There is statistically significant difference of the Bcl-2 / Bax ratio between treating group compared with model group（ P 〈0-001）,which is higher than that of the former. There is not statistically difference of Bcl-2 / Bax ratio between treating group compared with healthy group（ P〈0. 05） Conclusions Ganshi Qingdai Cream can inhibit the Bcl-2 protein expression,promot the Bax protein expression,and make the cell apoptosis regulation system tend to balance.