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rhFGF-21的高效表达及其纯化工艺的优化

Fermentation and Purifcation Process Optimization Development of FGF-21
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摘要 成纤维细胞生长因子-21(FGF-21)是FGF家族的一员。现有研究表明,FGF-21是一种血糖调节因子,有望被开发成为治疗糖尿病的候选药物。本实验是在前期研究的基础上,对高效表达FGF-21基因的工程菌株进行接种、发酵、诱导表达,将表达产物纯化,PEG修饰提高其体内半衰期,通过改变反应体系的反应时间、反应温度、蛋白浓度和反应物之间的质量比优化修饰反应。阴离子交换层析纯化反应混合物,最终得到纯度大于98%的活性FGF-21蛋白。 Fibroblast growth factor 21（ FGF-21） is a member of the FGF family. The existing studies show that,FGF-21 is a blood sugar regulator and is expected to be developed as a drug for diabetes. The study is to express FGF-21 in sumo prokaryotic expression system based on the previous study. The expression product is purified,PEG-modified to improve its in vivo half-life. The reaction is optimized by varying the time,the temperature,protein concentration and the ratio of the reactants. The mixture was purified by ion exchange chromatography,and the purity of FGF-21 is over 98%.
作者 于聪 李剑 周长林
机构地区 中国药科大学生命科学与技术学院 天津天士力集团有限公司生物药品研究所
出处 《药物生物技术》 CAS 2015年第3期218-221,共4页 Pharmaceutical Biotechnology
关键词 成纤维细胞生长因子-21 亲和层析 sumo酶 离子交换 聚乙二醇化 糖尿病 Fibroblast growth factor 21 Affinity chromatography Sumo enzyme Ion exchange chromatography Polyethylene glycol Diabetes
分类号 Q51 [生物学—生物化学] Q786 [生物学—分子生物学]
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参考文献13

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