阿托伐他汀对瘦素诱导血管内皮细胞白细胞介素-8、白细胞介素-1β表达的影响

Impact of atorvastatin on expressions of interleukin-8 and interleukin-1β in leptin-induced endothelial cells

目的 探讨瘦素对血管内皮细胞（vascular endothelial cells,VECs）白细胞介素-8（interleukin-8,IL-8）和白细胞介素-1β（interleukin-1β,IL-1β）表达的作用及阿托伐他汀对其的干预影响。方法 取Sprague-Dawley（SD）雄性大鼠培养血管内皮细胞,取4~5代细胞用于实验。内皮细胞受100 ng/m L瘦素分别作用0、1、3、6、12 h;10μmol/L阿托伐他汀分别干预细胞0、1、3、6、12、24 h后用100 ng/m L瘦素干预6 h。运用酶联免疫吸附试验（ELISA）法检测各组细胞上清液IL-8、IL-1β浓度;实时荧光定量聚合酶链式反应（real-time quantitative polymerase chain reaction,rt-PCR）检测各组细胞上清液IL-8m RNA、IL-1βm RNA表达的量;噻唑蓝（MTT）法检测VEC增殖。结果 瘦素刺激组IL-8、IL-1β表达高于对照组,且随着瘦素作用时间延长IL-8、IL-1β浓度也随之增加。阿托伐他汀组IL-8、IL-1β的表达浓度低于对照组,且随着阿托伐他汀作用时间的延长IL-8、IL-1β浓度也随之降低,24 h抑制作用最明显。结论 瘦素可以增强血管内皮细胞中IL-8、IL-1β的表达,并成时间依赖性;阿托伐他汀可以降低瘦素诱导的IL-8、IL-1β的表达浓度。

Objectives To explore the effect of leptin on the expressions of interleukin-8（IL-8） and interleukin-1β（IL-1β） in vascular endothelial cells（VECs） and the role of atorvastatin in the intervention. Methods Male SpragueDawley（SD） rats were cultured for endothelial cells, and the 4-5 passage cells were used in the experiment.Endothelial cells were cultured by 100 ng / m L leptin for 0, 1, 3, 6, 12 h; and were intervened by 10 μmol / L atorvastatin for 0, 1, 3, 6, 12, 24 h, after that, cultured by 100 ng / m L leptin for 6 h. Enzyme-linked immunosorbent assay（ELISA） was used to detect cell supernatant for expressions of IL-8 and IL-1β in each group. Real-time quantitative polymerase chain reaction（rt-PCR） was used to detect the cell supernatant for expressions of IL-8m RNA and IL-1βm RNA. Methyl-thiazol yl-tetrazolium（MTT） assay was used to detect VEC proliferation. Results Expressions of IL-8and IL-1β in leptin-stimulation group were significantly higher than those in control group, and as the role of leptin prolonged, the expressions of IL-8 and IL-1β increased. Expressions of IL-8 and IL-1β in atorvastatin group were lower than those in control group, and as atorvastatin extended the duration of action, the expressions of IL-8 and IL-1βdecreased, the inhibition at 24 h was the most obvious. Conclusions Leptin can enhance expressions of IL-8 and IL-1βin VECs in a time-dependent manner. Atorvastatin can reduce expressions of IL-8 and IL-1β in leptin-induced VECs.