钴-60辐照对补骨脂药材指纹图谱的影响

Influence of 60 ^Co-γ Irradiation on Fingerprint of Psoralea Corylifolia

目的：通过高效液相色谱（HPLC）法建立补骨脂药材的指纹图谱,研究不同剂量钴-60（60Co-γ）射线辐照对有效成分的影响。方法：利用HPLC法,以Shim-Pack VP-ODS（4.6 mm×250 mm,5μm）色谱柱作分离柱;乙腈、水为流动相作梯度洗脱,检测波长为246 nm。结果：确定了8个共有峰,不同剂量辐照前后补骨脂药材指纹图谱的相似度为0.999~1.000,且方法学考察符合规定的标准。结论：不同剂量辐照前后补骨脂药材有效成分无明显变化。

Objective： Establish the fingerprint of psoralea corylifolia by high performance liquid chromatography （HPLC）, to study the influence of different doses 60 Co-γ irradiation on the effective components. Methods： Carring out the method for HPLC. We use Shim- Pack VP-ODS （4.6 mm×250 mm,5μm） column as detached dowel and a mixture of acetonitrile and water as mobile phase for gradient elution, the detection wavelength was set at 246 rim. Results： Fingerprint was established with 8 common peaks, the similarity was in the range of 0. 999 - 1. 000, and methodology reached the required standard. Conclusion：The effective ingredients of fructus psoralea had no obvious change before and after 60 ^Co-γ irradiation with different doses.